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Table 3 DNA glycosylase activity of N. meningitidis (Mc) recombinant Fpg protein.

From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair

Substrate

Released bases (fmol)

 

Average

(St. dev.)c

N. meningitidis Fpga

75

(± 30)

E. coli Fpgb

64

(± 44)

No enzyme

12

(± 4)

  1. a 500 ng of protein was employed in each reaction
  2. b 160 Units of protein was employed in each reaction
  3. c standard deviation of the mean
  4. Removal of formamidopyrimidine (faPy) from [3H]-methyl-faPy-poly(dG·dC) DNA by recombinant Mc and E. coli Fpg. The results are given as the average of five independent measurements.
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BMC Microbiology

ISSN: 1471-2180

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