From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair
Plasmid, strains | Relevant characteristic | Source/Reference |
---|---|---|
Plasmids | ||
pET22b | Expression vector, T7 promoter-driven system, His-tag, ampR | Novagen |
pET22b-fpg M1080 | pET22b harbouring fpg from Mc M1080 | This study |
pARR2107 | Contains an Universal Rate Of Switching cassette | [22] |
pUD | pARR2107 harbouring a 12-mer DUS | This study |
Strains | ||
Escherichia coli | ||
ER2566 | Expression host with chromosomal copy of the T7 | New England |
RNA polymerase gene | Biolabs | |
ER2566-pET22b-fpg M1080 | ER2566 expressing Mc M1080 fpg from pET22b | This study |
Neisseria meningitidis | ||
M1080 | Serogroup B, isolated in the USA in 1970 | [45] |
Z1099 | Serogroup A, isolated in the Philippines in 1968 | Dominique A. Caugant |
NmZ1099_UROS | Z1099 harbouring a Universal Rate Of Switching cassette | This study |
NmZ1099_UROSΔfpg | Z1099 fpg strain harbouring a Universal Rate Of Switching cassette | This study |
NmZ1099_UROSΔmutS | Z1099 mutS strain harbouring a Universal Rate Of Switching cassette | This study |