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Table 1 Bacterial strains and plasmids used in this study.

From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repair

Plasmid, strains

Relevant characteristic

Source/Reference

Plasmids

  

pET22b

Expression vector, T7 promoter-driven system, His-tag, ampR

Novagen

pET22b-fpg M1080

pET22b harbouring fpg from Mc M1080

This study

pARR2107

Contains an Universal Rate Of Switching cassette

[22]

pUD

pARR2107 harbouring a 12-mer DUS

This study

Strains

  

Escherichia coli

  

ER2566

Expression host with chromosomal copy of the T7

New England

 

RNA polymerase gene

Biolabs

ER2566-pET22b-fpg M1080

ER2566 expressing Mc M1080 fpg from pET22b

This study

Neisseria meningitidis

  

M1080

Serogroup B, isolated in the USA in 1970

[45]

Z1099

Serogroup A, isolated in the Philippines in 1968

Dominique A. Caugant

NmZ1099_UROS

Z1099 harbouring a Universal Rate Of Switching cassette

This study

NmZ1099_UROSΔfpg

Z1099 fpg strain harbouring a Universal Rate Of Switching cassette

This study

NmZ1099_UROSΔmutS

Z1099 mutS strain harbouring a Universal Rate Of Switching cassette

This study