Figure 2From: Characterization of the meningococcal DNA glycosylase Fpg involved in base excision repairDNA glycosylase activity of N. meningtidis (Mc) recombinant Fpg protein. (A) 1 ng of purified Mc Fpg or 0.032 Units of E. coli Fpg was incubated with 10–50 fmol of a 24 bp duplex oligodeoxyribonucleotide containing a single 8oxoG residue opposite A, T C or G. Base excision and strand cleavage were analysed by 20% PAGE and phosphorimaging. The arrow indicates the cleaved DNA substrate. * denotes 32P-labelled strand. S; substrate. (B) Quantification of strand cleavage activity by Mc Fpg. The results represent the average of three independent experiments and error bars indicate the standard deviation of the mean.Back to article page