Skip to main content
Figure 1 | BMC Microbiology

Figure 1

From: Characterization of the hupSL promoter activity in Nostoc punctiformeATCC 29133

Figure 1

hupSL and its promoter region in Nostoc punctiforme ATCC 29133. Detailed view of the nucleotides in the hupSL promoter region. Putative binding sites for regulatory proteins (IHF and NtcA), the transcription start point and the -35 and the – 10 boxes are marked [14]. Primers used for gel shift assay (see Fig. 2) are shown as arrows in the figure. Below the hupSL promoter sequence, the intergenic region between Npun_R0367 and hupS together with hupS are shown (WT). Furthermore, the five promoter deletion constructs, where truncated versions of the hupSL promoter have been coupled to gfp or luxAB, are also shown (A to E). Total length of the promoter fragments and starting position relative to transcription start point are indicated. The grey lines symbolise the hupSL promoter sequence, and the white lines symbolise the DNA sequence belonging to the vector used for the constructs, pSUN202.

Back to article page