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Table 1 Primers used in this study.

From: Diversity and transcription of proteases involved in the maturation of hydrogenases in Nostoc punctiforme ATCC 29133 and Nostocsp. strain PCC 7120

Strain/Target

Oligonucleotide (name and sequence), 5'→3'

Primer pair

Product size (bp)

Nostoc punctiformeATCC 29133

RT-Reaction

    

hupW- antisense

HupW N R

TCA CAT CAT CGG GAA AGT CA

  

Subsequent PCR

    

hupW-antisense

HybD-RACE 1

TTC TGG CAA AGC TTC CAG TT

L0373/L0372

808/1361

Npun_F0373-sense

L0373

AAT TAT CTC CCT CGC GTT CC

HybD-RACE 1

808

NpF0372-sense

L0372

TTG CCG ATG AAA CAA ATG AA

HybD-RACE 1

1361

Northern blot, probe

    

hupW-antisense

HupW N R

TCA CAT CAT CGG GAA AGT CA

NB hupW N

336

hupW-sense

NB hupW N L

TTG GTT GCG GAA ATC TCA AT

HupW N R

336

5'RACE

    

cDNA synthesis

HybD-RACE 1

TTC TGG CAA AGC TTC CAG TT

  

1 st PCR

HybD-RACE 2

TGT TGG GCA ATG ATT ACA CCT

  

2 nd PCR

HybD-RACE 3

ATT GAG ATT TCC GCA ACC AA

  

Nostoc sp. strain PCC 7120 – hupW

RT-Reaction

    

hupW- antisense

NB HupW- AR

TGC TGT AGG CGT AAT CAT CG

  

Subsequenct PCR

    

hupW-antisense

Alr1422-23 R

TTT GTA AGC GTT GAG CGA TG

Alr1422-23 L

490

Alr1422-sense

Alr1422-23 L

ACC GAA CTC CGC AGA AAC TA

Alr1422-23 R

490

5'RACE

    

cDNA synthesis

ALR1423 RACE 1b

GTT CCG AAC CAG TGG AAC TC

  

1 st PCR

ALR1423 RACE 2

TTT GTA AGC GTT GAG CGA TG

  

2 nd PCR

ALR1423 RACE 3

GAG ATT TCC GCA ACC GAT AA

  

Nostoc sp. strain PCC 7120 – alr1422

5'RACE

    

cDNA synthesis

5-1422-1

CCTAAAGTCGGTGGAAAATCGGC

  

1 st PCR

5-1422-2

TTCTTCCGTGACAAATCGTG

  

2 nd PCR

5-1422-3

TTTTTGATGGACGGATGACA

  

Nostoc sp. strain PCC 7120 – hoxW

Northern blot, probe

    

hoxW-antisense

NB HoxW A R

AAA GCG ATC GCC TAT TTC AA

HoxW L

316

hoxW-sense

HoxW L

AGG ACA ACG GAT AGC GAA TG

NB HoxW A R

316

5'RACE

    

cDNA synthesis

5'RACE-1 HoxW/A

CAC AGC ACG ACG AAC AAG GCT CCA ACT TCA AAC CA

  

1 st PCR-TAG

5'RACE-TAG Hox/A

CAC AGC ACG ACG AAC AAG G

5'RACE-polyG Hox/A

 

1 st PCR-PolyG

5'RACE-polyG Hox/A

CAC AGC ACG ACG AAC AAG GGG GGG GGG GG

5'RACE-TAG Hox/A

 
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BMC Microbiology

ISSN: 1471-2180

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