Figure 3

The effect of RpoE on SsrB-regulated genes is downstream of ssrAB expression. The ssrB gene in wild type and ΔrpoE cells was replaced with an ssrB-FLAG allele in its native location on the chromosome. Cells were grown under SsrB-activating conditions for six hours and lysates were probed by western blot to detect SsrB-FLAG and intracellular DnaK as a control. The data shown are representative of two experiments performed independently with identical results.