Figure 2
From: Quelling targets the rDNA locus and functions in rDNA copy number control
Detection of NTS siRNAs from immunoprecipitated QDE2 protein. The western blot analysis (WB) on the immunoprecipitation using anti-FLAG antibody shows a signal corresponding to QDE2 protein only in the strain that express the tagged version of QDE2 (QDE2FLAG) and not in the control strain in which the qde2 genes is deleted (qde2-). The northern blot analysis on RNA extracted from the immunoprecipitate shows a specific signal corresponding to anti-sense NTS siRNAs only in the strain that expresses the tagged version of QDE2 (QDE2FLAG). A signal corresponding to siRNAs derived from the silenced Al-1 locus is shown as a control of the experiment.