Table 1 Bacterial strains, plasmids and primers

E. coli

DH5α

supE44 Δ(lacZYA-argF) U169 (Δ80lacZ ΔM15) hsdR17 recA endA1 gyrA96 thi-1 relA1

Gibco

Salmonella enterica serovar Typhimurium

JSG210

ATCC 14208 (CDC6516-60), wild type

ATCC

JSG1998

JSG210 ΔpreA1998

[3]

JSG2343

JSG210 ΔpreB2343

[3]

JSG2626

JSG210 ΔpreAB2626

[3]

JSG1225

fliA::Tn10 dTet

gift of K. Klose

JSG648

phoN::cam prgH1::TnphoA

[7]

Plasmids

P_BAD18

ColE1 ori, pBAD L-Ara inducible (Ap^r)

[9]

pRK2013::Tn7

ColE1 mob Δtra RK2 Δrep RK2 repE kan::Tn7 (Tp^r Sm^r Sp^r)

[8]

pJSG2558

P_BAD18 with a 0.7-kb fragment containing preA expressed from pBAD (Ap^r)

[3]

pJSG2581

P_BAD18 with a 1.5-kb fragment containing preAB expressed from pBAD (Ap^r)

[3]

Primers (5'-3')

6-FAM-ccatcgccaataagtgtgtc

preA Reverse (primer ext.)

This study

6-FAM-cagggtgtcattcaactggc

mdaB Reverse (primer ext.)

This study

6-FAM-gatgacgctcaatgtggtcg

STM3175 Reverse (primer ext.)

This study

6-FAM-ttcgcaaactggtcgaggac

ygiN Reverse (primer ext.)

This study

6-FAM-tgatcacgtacatggagtag

parC Reverse (primer ext.)

This study

6-FAM-gtagaacacagtgccataac

ygiW Reverse (primer ext.)

This study

ggtagaacacagtgccataac

preA F (primer ext.)

This study

ctcggtaaaccagtcgacgc

preA R (primer ext.)

This study

ggaaggtggatttgaggc

mdaB F (primer ext.)

This study

gcagcttcaccgtcagagata

mdaB F (primer ext.)

This study

gacgatcttaacctgatgacc

mdaB R (primer ext.)

This study

cgaagtggataaagactggaac

STM3175 F (primer ext.)

This study

tagcgatagagcggaagc

STM3175 R (primer ext.)

This study

gcgtctatctgccattcc

ygiN F (primer ext.)

This study

gcggcatgatccaccatc

ygiN R (primer ext.)

This study

cctgaatttcgtccatgagg

parC F (primer ext.)

This study

gaatagcgagattcctggcg

parC F (primer ext)

This study

ccagctctgacatcgcatag

parC R (primer ext.)

This study

ccatcgccaataagtgtgtc

ygiW F (primer ext.)

This study

cgtcacgcagcgatttagc

ygiW R (primer ext.)

This study

ggccgaacactctttgtggt

dnaN F (real-time)

This study

gtataatttcggtcgcatccgt

dnaN R (real-time)

This study

atatcgtcgagcgcatttcc

ygiW F (real-time)

This study

tccagtctttatccacttcgcc

ygiW R (real-time)

This study

aagagttcgcgttgctggaa (JG1134)

preA F (real-time, RT-PCR)

This study

gagcttgcggcgtaaatgat

preA R (real-time)

This study

agactctggcgcctgactcg

ygiN F (real-time)

This study

aacgccggattccagaatacg

ygiN R (real-time)

This study

acaggcttaagagtagcggctg (JG1137)

preB R (RT-PCR)

This study

atatcgtcgagcgcatttcc (JG1132)

ygiW F (RT-PCR)

This study

cgcggatccttaacgaagcggcagatagatatc (JG1223)

STM 3175 R(RT-PCR)

This study

gtgtcgtttggcaacgccgcggaa (JG1703)

preB F(RT-PCR)

This study

caactggccgttggagtgcgcg (JG1704)

mdaB R (RT-PCR)

This study

tgccggatgttccgcgctataccgca (JG1705)

mdaB F (RT-PCR)

This study

tgacggtgatgttggcccggacgcg (JG1706)

ygiN R (RT-PCR)

This study

gaagccgtccagcagttg (JG1861)

STM 1595 F (Real-time PCR)

This study

gcgataaccattccaccaaac (JG1862)

STM 1595 R (Real-time PCR)

This study

cgttcctaaacttgcgttacag (JG1863)

STM 3175 F (Real-time PCR)

This study

gctggcgttgaccttatcc (JG1864)

STM 3175 R (Real-time PCR)

This study

ttgtatctggagattgtggactac (JG1865)

STM 1685 F (Real-time PCR)

This study

gagcccgtcgcaaagttg (JG1866)

STM 1685 R (Real-time PCR)

This study

tctacgcttgttcgcttac (JG1867)

STM 1252 F (Real-time PCR)

This study

ggtgttgtccagatattatgttc (JG1868)

STM 1252 R (Real-time PCR)

This study

tacagtggacaatgaatg (JG1869)

STM 1684 F (Real-time PCR)

This study

gctatggctatgtaacag (JG1870)

STM 1684 R (Real-time PCR)

This study

ggcttcacggcggcaatg (JG1871)

STM 2080 F (Real-time PCR)

This study

tcacgatacgggagggataaagg (JG1872)

STM 2080 R (Real-time PCR)

This study

ctaacttccaggaccactc (JG1873)

STM 4118 F (Real-time PCR)

This study

gataaccgtacagactcatac (JG1874)

STM 4118 R (Real-time PCR)

This study

tgatatgggcgttctggtctg (JG1875)

STM 1253 F (Real-time PCR)

This study

cgtgctgccagtgaggag (JG1876)

STM 1253 R (Real-time PCR)

This study