Figure 2

RT-PCR analysis of the dnd genes transcripts. dnd gene transcripts were reverse transcribed and amplified. (A) Relative positions and directions of corresponding primers are marked with black arrows. (B) Amplification products with sense primer (SP), anti sense primer (AP) and their corresponding lengths. Intra-dnd gene amplification products are indicated as dnd gene names, while products of regions between dnd genes are named linking two corresponding genes such as AB. Amplification of 16S rRNA is used as an internal control marker (IM). (C) Electrophoresis of RT-PCR products. The amplification products are labeled as in Figure 2B. Reverse transcriptase inactivation (BC*) and without DNase treatment (AB') were carried out as negative and positive controls. DNA markers are labeled as "M".