- Research article
- Open Access
NF-κB activation by Helicobacter pylorirequires Akt-mediated phosphorylation of p65
© Takeshima et al; licensee BioMed Central Ltd. 2009
Received: 13 September 2008
Accepted: 12 February 2009
Published: 12 February 2009
This article has been retracted. The retraction notice can be found here: http://0-bmcmicrobiol.biomedcentral.com.brum.beds.ac.uk/articles/10.1186/1471-2180-11-128
The Retraction Note to this article has been published in BMC Microbiology 2011 11:128
The inflammatory response in Helicobacter pylori-infected gastric tissue is mediated by cag pathogenicity island (PAI)-dependent activation of nuclear factor-κB (NF-κB). Phosphatidylinositol 3-kinase (PI3K)/Akt signaling is known to play a role in NF-κB activation, but little information is available on the relationship between H. pylori and PI3K/Akt signaling in gastric epithelial cells. We examined whether H. pylori activates Akt in gastric epithelial cells, the role of cag PAI in this process and the role of Akt in regulating H. pylori-induced NF-κB activation.
Phosphorylated Akt was detected in epithelial cells of H. pylori-positive gastric tissues. Although Akt was activated in MKN45 and AGS cells by coculture with cag PAI-positive H. pylori strains, a cag PAI-negative mutant showed no activation of Akt. H. pylori also induced p65 phosphorylation. PI3K inhibitor suppressed H. pylori-induced p65 phosphorylation and NF-κB transactivation, as well as interleukin-8 expression. Furthermore, transfection with a dominant-negative Akt inhibited H. pylori-induced NF-κB transactivation. Transfection with small interference RNAs for p65 and Akt also inhibited H. pylori-induced interleukin-8 expression.
The results suggest that cag PAI-positive H. pylori activates Akt in gastric epithelial cells and this may contribute to H. pylori-mediated NF-κB activation associated with mucosal inflammation and carcinogenesis.
Helicobacter pylori causes various human gastric diseases. In 10 to 20% of infected individuals, H. pylori-induced chronic gastric inflammation progresses to gastroduodenal ulcers, gastric cancer or gastric mucosa-associated lymphoid tissue lymphoma [1, 2]. Bacterial, environmental and host genetic factors may affect the progress and outcome of gastric disease in these individuals. Virulence of individual H. pylori strains is one such factor responsible for severe disease, and several virulence factors have been described such as the presence of a cag pathogenicity island (PAI) and vacuolating cytotoxin (VacA) [3–6]. The presence of cag PAI genes correlates strongly with the development of ulcer diseases and gastric cancer [7–9].
Nuclear factor-κB (NF-κB) is a crucial regulator of many cellular processes, including immune response, inflammation and apoptosis . It has been established that inflammation plays an important role in cancer development . The five known mammalian Rel genes encode seven Rel-related proteins: RelA/p65; p105 and its processing product, p50; p100 and its processing product, p52; c-Rel; and RelB. Each contains an N-terminal Rel homology domain that mediates DNA binding, dimerization and interaction with the IκB family of NF-κB inhibitors. p65, c-Rel and RelB contain C-terminal transactivation domains, but p50 and p52 do not. The prototypical NF-κB complex is a p50–p65 heterodimer. In resting cells, NF-κB is complexed to cytoplasmic NF-κB inhibitors. IκBα is the best characterized of these inhibitors. NF-κB activation requires phosphorylation of two conserved serine residues within the N-terminal domain of IκBα (serines 32 and 36) . Phosphorylation leads to ubiquitination and 26S proteasome-mediated degradation of IκBα, thereby releasing NF-κB from the complex where it translocates to the nucleus and activates various genes. Stimulation such as cytokines results in the activation of specific intracellular signaling pathways with subsequent activation of the IκB kinase (IKK) complex. This complex comprises two catalytic subunits (IKKα and IKKβ) and the regulatory subunit (IKKγ), and can phosphorylate IκBα . Only H. pylori strains containing the cag PAI (cag PAI+) can direct signaling in gastric epithelial cells to activate the IKK complex and thus NF-κB, leading to the release of chemoattractants such as interleukin (IL)-8 . However, the exact mechanism by which cag PAI+ H. pylori strains induce activation of NF-κB in gastric epithelial cells is not clear yet. The cag PAI encodes a bacterial type IV secretion capable of translocating effector molecules . Based on the observations that mutants of CagA, the only type IV secretion system effector protein, often induce a considerable amount of IL-8, early studies reported that CagA did not activate NF-κB or IL-8 secretion in infected cells [15, 16]. However, CagA was recently reported to induce IL-8 release through NF-κB activation in time- and strain-dependent manners .
Protein kinases are also required for optimal NF-κB activation by targeting functional domains of NF-κB protein itself. Phosphorylation of the p65 subunit plays a key role in determining both the strength and duration of the NF-κB-mediated transcriptional response [18, 19]. Sites of phosphorylation reported to date are serines 276 and 311, in the Rel-homology domain, and serines 468, 529 and 536, three phosphoacceptor sites located in the transactivation domain. Importantly, phosphorylation at serine 536 reduced the ability of p65 to bind IκBα  and facilitated the recruitment of TAFII31, a component of the basal transcriptional machinery . Phosphorylation at serine 536 is also responsible for recruiting coactivators such as p300 . The above data emphasize the importance of p65 phosphorylation at serine 536 in the function of NF-κB. In contrast, p50 phosphorylation does not regulate NF-κB activation, because p50 lacks a transactivation domain. Akt is a downstream effector of phosphatidylinositol 3-kinase (PI3K) that has been implicated in phosphorylation of serine 536 on the p65 subunit [18, 19]. Akt activation also mediates multiple biological activities including increased survival, proliferation and growth of tumor cells. The present study investigated whether Akt regulates NF-κB activation in response to H. pylori infection.
Cag PAI is required for H. pylori-mediated IL-8 induction in gastric epithelial cells
H. pyloriactivates Akt and induces phosphorylation of the NF-κB p65 subunit in gastric epithelial cells
We next examined whether the observed Akt activation was specific to the cag PAI domain, based on the above results indicating the importance of cag PAI expression for IL-8 induction in gastric epithelial cells in vitro (Figure 2). We used a wild-type H. pylori strain (26695) and an isogenic cag PAI mutant (Δcag PAI). Stimulation with the wild-type strain induced Akt phosphorylation in MKN45 and AGS cells, while the isogenic mutant that lacked the expression of cag PAI did not (Figure 3B). These results suggest the important role of H. pylori cag PAI in the phosphorylation of Akt.
H. pylori-induced p65 phosphorylation is PI3K-dependent
H. pylori-stimulated NF-κB transcriptional activity is dependent on PI3K/Akt
PI3K inhibition or transfection with small interference RNAs for p65 and Akt suppresses H. pylori-induced IL-8 expression
NF-κB activation is known to regulate various cellular responses, including apoptosis, and is required for the induction of inflammatory and tissue-repair genes . As reported previously [26, 28], we demonstrated that H. pylori modulates the NF-κB system in gastric epithelial cells by inducing IκBα phosphorylation and degradation, NF-κB DNA binding activity, and NF-κB transcriptional activity. Although this investigation was a preliminary in nature in a small number of patients, we also showed that H. pylori infection activated Akt in epithelial cells, both in vivo and in vitro, and that this is dependent on an intact cag PAI in vitro. Interestingly, H. pylori also stimulated endogenous p65 phosphorylation on serine 536. Phosphorylation of p65 at serine 536 in the transactivation domain enhances the transcriptional activity of NF-κB . Although previous studies using pathogenic strains containing the cag PAI showed NF-κB activation and cytokine expression in gastric epithelial cells [13, 28], ours is the first demonstration that cag PAI+ H. pylori strains induce gene expression through p65 phosphorylation, and that H. pylori-induced p65 phosphorylation is PI3K/Akt-dependent.
The role of PI3K/Akt signaling cascades in the regulation of NF-κB transactivation remains controversial. The present study agrees with previous investigators in demonstrating that activation of PI3K/Akt promotes the activation of p65 , while some others found that inhibition of the PI3K/Akt pathway augmented p65 activation . We also analyzed how H. pylori-stimulated PI3K activation leads to the activation of NF-κB, and identified a pathway initiated by the PI3K activation that is distinct from NF-κB DNA binding. In contrast to the lack of effect of inhibition of PI3K on NF-κB DNA binding, pretreatment of MKN45 cells with LY294002 resulted in marked inhibition of H. pylori-stimulated p65 phosphorylation and the ability of H. pylori to activate NF-κB-dependent transcription. Furthermore, a dominant-negative derivative of Akt blocked the ability of H. pylori to activate an NF-κB-dependent promoter. Therefore, the results established a clear role of PI3K and its downstream effector Akt in modulating the transactivation potential of p65. However, the kinases and signaling pathway responsible for H. pylori-induced p65 phosphorylation remain unknown. Our data demonstrated for the first time that PI3K and Akt participate in H. pylori-mediated NF-κB transcriptional activity. Further studies are required to define the exact signaling cascade involved in bacteria-induced p65 phosphorylation and NF-κB activity.
Our data demonstrated the role of PI3K/Akt in H. pylori-induced NF-κB transcriptional activity and subsequent IL-8 production in gastric epithelial cells. We also demonstrated an important role of PI3K/Akt in the regulation of gastric responses to H. pylori infection, thereby elucidating a novel mechanism that controls both transcription and gene expression in bacterial pathogenesis.
Antibodies and reagents
Polyclonal antibodies to Akt, phospho-Akt (threonine 308), phospho-Akt (serine 473), p65 and phospho-p65 (serine 536), as well as monoclonal antibodies to phospho-Akt (serine 473) and phospho-IκBα (serines 32 and 36) were purchased from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibodies to IκBα and NF-κB subunits p50, p65, c-Rel, p52 and RelB were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal antibody to actin was purchased from NeoMarkers (Fremont, CA, USA). PI3K inhibitor LY294002 was obtained from Calbiochem (La Jolla, CA, USA).
H. pylori ATCC 49503 (American Type Culture Collection, Rockville, MD, USA) was used. Isogenic H. pylori mutants lacking the cag PAI , VacA and virD4 were also studied together with their parental wild-type strain (26695). Isogenic null mutants derived from 26695 were constructed by insertional mutagenesis, using aphA (conferring kanamycin resistance). H. pylori strains were plated on blood agar plates and incubated at 37°C for 2 days under microaerophilic conditions. Using inoculating needles, bacteria harvested from the plates were suspended in 50 ml of brucella broth containing 5% fetal bovine serum (FBS) and then cultured in a liquid medium at 37°C for 1 day in a controlled microaerophilic environment. Bacteria were harvested from the broth culture by centrifugation and then resuspended at the concentrations indicated below in antibiotic-free medium. All procedures were approved by the appropriate institutional biosafety review committees and were conducted in compliance with biohazard guidelines.
The human gastric epithelial cell lines MKN45 and AGS were maintained in RPMI 1640 containing 10% FBS and antibiotics. On the day of the experiment, cells were plated on fresh serum- and antibiotic-free medium and cocultured with H. pylori at a final concentration of 107 colony forming unit/ml for the times indicated below.
We examined stomach biopsy specimens from 10 patients with H. pylori gastritis and three histopathologically-normal stomach biopsies. We analyzed the phosphorylation status of Akt at serine 473 and the presence of H. pylori infection by culture, serological analysis (with anti-H. pylori IgG antibody), rapid urease test and histological visualization with Giemsa staining. Patients with H. pylori gastritis showed polymorphonuclear neutrophil infiltration in the gastric epithelium in conjunction with bacteria consistent with H. pylori. All subjects provided informed consent before obtaining the biopsy samples.
Specific primers used in RT-PCR
Product size (bp)
The Akt dominant-negative mutant plasmid (pCMV5-K169A, T308A, S473A-Akt) encodes lysine169 (the ATP-binding site), threonine 308 and serine 473 (the phosphorylation sites) to alanine mutations. Reporter plasmid κB-LUC is a luciferase expression plasmid controlled by five tandem repeats of the NF-κB-binding sequences of the IL-2 receptor (IL-2R) α chain gene.
Transfection and luciferase assay
MKN45 cells were transfected with 1 μg of the appropriate reporter plasmid and 5 μg of effector plasmid using Lipofectamine (Invitrogen). After 24 h, H. pylori was added at a ratio of bacteria to cells of 20:1 and incubated for another 24 h. Luciferase activities were measured using the dual luciferase assay system (Promega, Madison, WI, USA) and normalized by the renilla luciferase activity from phRL-TK.
Preparation of nuclear extracts and EMSA
Cell pellets were swirled to a loose suspension and treated with lysis buffer (0.2 ml, containing 10 mM HEPES, pH 7.9, 10 mM KCl, 0.1 mM EDTA, 0.1 mM EGTA, 2 mM AEBSF and 1 mM DTT) with gentle mixing at 4°C. After 10 min, NP40 was added to a final concentration of 0.8% and the solution was immediately centrifuged for 5 min at 700 rpm at 4°C. The supernatant was removed carefully and the nuclei diluted immediately by the addition of lysis buffer without NP40 (1 ml). The nuclei were then recovered by centrifugation for 5 min at 700 rpm at 4°C. Finally, the remaining pellet was suspended on ice in the following extraction buffer (20 mM HEPES, pH 7.9, 0.4 M NaCl, 1 mM EDTA, 1 mM EGTA, 1 mM DTT, 2 mM AEBSF, 33 μg/ml aprotinin, 10 μg/ml leupeptin, 10 μg/ml E-64 and 10 μg/ml pepstatin A) for 30 min to obtain the nuclear fraction. All fractions were cleared by centrifugation for 15 min at 15,000 rpm. NF-κB binding activity with the NF-κB element was examined by EMSA as described previously . In brief, 5 μg of nuclear extracts were preincubated in a binding buffer containing 1 μg poly(dI-dC)·poly(dI-dC) (Amersham Biosciences, Piscataway, NJ, USA), followed by the addition of a radiolabeled oligonucleotide probe containing NF-κB element from the IL-2R α chain gene (approximately 50,000 cpm). The radiolabeled oligonucleotide was prepared by filling in the overhang with the Klenow fragment of DNA polymerase I in the presence of 32P-dCTP and 32P-dATP. These mixtures were incubated for 15 min at room temperature. The DNA protein complexes were separated on a 4% polyacrylamide gel and visualized by autoradiography. For competition experiments, the cold oligonucleotide probe or competitors were used, and supershift analysis was performed using antibodies against p50, p65, c-Rel, p52 or RelB. The probe or competitors used were prepared by annealing the sense and antisense synthetic oligonucleotides as follows: for the NF-κB element of the IL-2R α chain gene, 5'-GATCCGGCAGGGGAATCTCCCTCTC-3'; for the NF-κB element of the CCL20 gene, 5'-GATCGATCAATGGGGAAAACCCCATGTG-3'; and for the AP-1 element of the IL-8 gene, 5'-GATCGTGATGACTCAGGTT-3'. The above underlined sequences are the NF-κB and AP-1 binding sites.
Western blot analysis
Cells were lysed in a buffer containing 62.5 mM Tris-HCl, pH 6.8, 2% sodium dodecyl sulfate, 10% glycerol, 6% 2-mercaptoethanol and 0.01% bromophenol blue. Equal amounts of protein (20 μg) were subjected to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, followed by transfer to a polyvinylidene difluoride membrane and sequential probing with the specific antibodies. The enhanced chemiluminescence kit (GE Healthcare, Buckinghamshire, UK) was used for detection. The membranes were stripped in stripping buffer for probing with a different antibody. Actin served as an internal control in the Western blot procedure.
Akt kinase assay
A non-radioactivity-based Akt kinase assay kit was purchased from Cell Signaling Technology. After immunoprecipitation of Akt, the kinase reaction was performed using the instructions provided by the manufacturer with glycogen synthase kinase (GSK)-3 fusion protein as an exogenous substrate. The kinase reaction was analyzed by immunoblotting, using an anti-phospho-GSK-3 antibody (serines 21 and 9).
Measurement of IL-8 production
MKN45 cells were cultured in RPMI 1640 supplemented with 10% FBS in 24-well plates. Subconfluent monolayers of cells were cocultured with H. pylori for 24 h. The supernatants were collected and stored at -80°C. IL-8 was measured by ELISA (BioSource, Camarillo, CA, USA).
The siGENOME mixtures for p65 and Akt were obtained from Dharmacon (Chicago, IL, USA). All siRNA transfections were performed using a MicroPorator (Digital Bio, Seoul, Korea), pulsed once at 1,100 V for 20 ms. The siGENOME non-targeting siRNA served as controls.
Serial sections were deparaffinized in xylene and dehydrated using graded ethanol solutions. For better detection, sections were pretreated with ready-to-use proteinase K (Dako, Carpentaria, CA, USA) for 10 min at 37°C. This procedure increased the number of antigenic sites available for binding by the antibody. In the next step, the tissues were placed in 3% hydrogen peroxide and absolute methanol for 5 min to reduce endogenous peroxidase activity, followed by washing in PBS. Primary antibody incubations included anti-phospho Akt (serine 473) monoclonal antibody or a control IgG. After washing with PBS, the sections were covered with EnVision plus (Dako) for 40 min at 37°C and washed in PBS. Antigenic sites bound by the antibody were identified by reacting the sections with a mixture of 0.05% 3,3'-diaminobenzidine tetrahydrochloride in 50 mM Tris-HCl buffer and 0.01% hydrogen peroxide. Sections were counterstained with methyl green.
The authors thank T. Hirayama for providing H. pylori strain (ATCC 49503); J. Fujisawa for providing reporter plasmid κB-LUC; and D. R. Alessi for providing the dominant negative mutant of Akt. This work was supported in part by the Takeda Science Foundation and Grants-in-Aid for Scientific Research on Priority Areas from Ministry of Education, Culture, Sports, Science and Technology (20012044) and Scientific Research (C) from Japan Society for the Promotion of Science (19591123).
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