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Table 2 Primer sequences and annealing temperatures (Real Time PCR)

From: Inducible expression of beta defensins by human respiratory epithelial cells exposed to Aspergillus fumigatusorganisms

Primers

Sequences

Conditions

hBD2f

hBD2r

5'-tatctcctcttctcgttcctcttc-3'

5'-ccacaggtgccaatttgtttatac-3'

40 cycles, 55°C,

2.5% DMSO

hBD9f

hBD9r

5'-ggcctaaatccaggtgtgaa-3'

5'-tcaaatgttggcaagtggag-3'

40 cycles, 55°C

GAPDHf

GAPDHr

5'-acccactcctccacctttgac-3'

5'-tccaccaccctgttgctgtag-3'

40 cycles, 55°C