Table 4 Plasmids used in this work.
From: Characterisation of porin genes from Mycobacterium fortuitumand their impact on growth
Plasmids | Characteristics | Reference |
|---|---|---|
pIV2 | cloning vector with an origin of replication functional in Enterobacteriacea and a kanamycin resistance gene | [39] |
pLitmus38 | cloning vector with the origin of replication from pUC, an ampicillin resitance gene and the lacZ' gene for blue/white selection | New England Biolabs |
pMV306 | cloning vector replicating in E. coli with the kanamycin resistance gene aph from transposon Tn903 and the gene for the integrase and the attP site of phage L5 for integration into the mycobacterial genome | [40] |
pMV261 | Mycobacterium/E. coli shuttle vector with the kanamycin resistance gene aph from transposon Tn903 and the promoter from the hsp60 gene from M. tuberculosis | [40] |
pSHKLx1 | Mycobacterium/E. coli shuttle vector with a kanamycin resistance gene from Tn5, a hygromycin resistance gene and the promoter from the hsp60 gene from M. tuberculosis | [43] |
pSSa100 | pMV306 with a 3429 bp genomic DNA fragment from M. smegmatis SMR5 carrying mspA | [13] |
pSSp107, pSSp108 | pIV2 with a 2895 bp genomic DNA fragment from M. fortuitum 10860/03 carrying the porM1 gene | This study |
pSRb101 | pMV261 carrying the porM1 gene from M. fortuitum 10860/03 | This study |
pSRb103 | pMV261 carrying the porM2 gene from M. fortuitum 10851/03 | This study |
pSRa102 | pMV306 carrying the porM1 gene from M. fortuitum 10860/03 | This study |
pSRa104 | pMV306 carrying the porM2 gene from M. fortuitum 10851/03 | This study |
pSRr106 | pSHKLx1 carrying a 100 bp genomic DNA fragment from M. fortuitum 10860/03 containing the beginning of the porM1 gene with the SD-sequence in antisense-orientation with respect to the hsp60 promoter | This study |