Table 1 Primers and probes used in this study.
From: Characterisation of porin genes from Mycobacterium fortuitumand their impact on growth
Gene | Primers or probes | Sequence 5'-3' |
|---|---|---|
mspA | hpor | 5'-CGG TCT CAG CGA CCG AAC-3' |
| Â | npor | 5'-CCG GCG ATA CAG TTA GGG A-3' |
porM1 | mf-4IV-fw | 5'-TCT CCA GGG GCT GCT TTT G-3' |
| Â | mf-4-bw | 5'-CGG GAC GCC AAC CAC ATA AC-3' |
porM1 | komf-3f | 5'-CTG AAG CTT CAC CGA GCT GAG CAT CCT CAC-3' |
| Â | komf-4b | 5'-GAC ACT AGT CGT TGG CTA CAG AAC AAC ATT CC-3' |
porM2 | porM2-51-fw | 5'-ATG AAG GCA TTC AGT CGG G-3' |
| Â | porM2-51-bw | 5'-TGC TCC TCA AAG GAG AAG CG-3' |
porM2 | porM2-rev-1 | 5'-CGC TTC TCC TTT GAG GAG CA-3' |
| Â | porM2-rev-2 | 5'-TCC AGA CCC GCA TGA GAT ACG-3 |
porM2 | porM2-fw-hind | 5'-ACA AGC TTC AGC AAC GCT GTG AAC GCA-3' |
| Â | porM2-bw-hpa | 5'-CAG TTA ACA CTA CGG GAC GCT CGT GTC C-3' |
porM2 | porM2-rna-fw | 5'-CGC AAG CCT CTT CGT CGG C-3' |
| Â | porM2-rna-bw | 5'-CCA AGG TGC CCT CGA ACT CAT C-3' |
porM1 | porM1-as-1 | 5'-CGG ATC CTA GGG AGA ACA TGA AGG CAT TCA G-3' |
| Â | porM1-as-2 | 5'-CGG ATC CTT GTC CAG ACC CGC ATG AGA T-3' |
porM1 | porM1-fw-hind | 5'-ACA AGC TTG CTC TCA GCC GGT TTT CA-3' |
| Â | porM1-bw-hpa | 5'-CAG TTA ACG AAC TGG GCG TTC ATG TGC-3' |
porM1 | porM1-51-sybr-fw | 5'-GCT GTT TAC GAG CAC GGG C-3' |
| Â | porM1-51-sybr-bw | 5'-TTG CGG TCC AGG GGG AAC-3' |
mspA | mspATaqFW | 5'-CGT GCA GCA GTG GGA CAC CTT-3' |
| Â | mspATaqBW | 5'-CCA CGA TGT ACT TGG CGC GAC-3' |
| Â | mspATaqProbe | 5'-FAM-TGG ACC GCA ACC GTC TTA CCC GTG AGT G-TAMRA-3' |
porM | mfpqPCRfw | 5'-CGT TCA GCA GTG GGA CAC CTT-3' |
| Â | mfpqPCRrev | 5'-CCA CGG TGT ACT TGG CCC GGC-3' |
| Â | mfpqPCRprobe | 5'-FAM-TGG ACC GCA ACC GGC TGA CCC GTG AGT G-TAMRA-3' |