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Table 1 Bacterial strains and plasmids used in this study

From: Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli

Strain/plasmid

Characteristic(s)a

Motility In DMEM

FASb

Reference

Strains

    

   E2348/69

Wild type EPEC O127:H6

+

+

[47]

   ICC171

E2348/69 ΔescF (KanR)

+/-

-

[14]

   EPEC ΔespADB

E2348/69 ΔespADB (KanR)

+

-

This study

   EPEC ΔfliI

E2348/69 Δfli (CmR)

-

+

This study

   EPEC ΔfliC

E2348/69 ΔfliC (CmR)

-

+

Grubman et al, submitted

   EPEC ΔfliI/escF

E2348/69 ΔfliI/escF (CmR, KanR)

-

-

This study

   EPEC ΔfliI/fliS

E2348/69 ΔfliI/fliS (CmR, KanR)

  

This study

   EPEC ΔsepL

E2348/69 ΔsepL (KanR)

  

[48]

Plasmids

    

   pKD46

Red Recombinase helper plasmid (AmpR)

N.Ac

N.A

[45]

   pKD3

Red Recombinase template plasmid (CmR)

N.A

N.A

[45]

   pKD4

Red Recombinase template plasmid (KanR)

N.A

N.A

[45]

   pTrc99A

High-copy number expression vector (AmpR)

N.A

N.A

[49]

   pFliC

Derivative of pTrc99A encoding fliC from EPEC E2348/69 (AmpR)

N.A

N.A

This study

   pFliCEscF

Derivative of pTrc99A encoding fliC and escF from EPEC E2348/69 (AmpR)

N.A

N.A

This study

   pCDNA3

Eukaryotic expression vector

N.A

N.A

Promega

  1. aKan, kanamycin; Cm, chloramphenicol; Amp, ampicillin.
  2. bFAS, Fluorescent actin staining test.
  3. cN.A., not applicable.