Table 1 Primers used in these study (5' to 3' sequence)
From: Pseudomonas aeruginosa vesicles associate with and are internalized by human lung epithelial cells
| Â | PA2939 |
|---|---|
expression forward | TTACCGGAATTCATGAGCAACAAGAACA |
expression reverse | AACGGCAAGCTTTTACTTGATGAAGTCG |
KO up forward | TGTAACTAGTATGGTCAGCACATGTTGCA |
KO up reverse | GCCAGGGATGCGGCGGAATTCGAGAGGGCGAGGGCG |
KO down forward | CGCCCTCGCCCTCTCGAATTCCGCCGCATCCCTGGC |
KO down reverse | CTGACCTCGAGTTACTTGATGAAGTCGTGAC |
| Â | Tet R cassette from pACYC184 |
EcoRI Tet forward | GGTTATGAATTCGGTAGCTCAGAGAACCCTTCG |
EcoRI Tet reverse | GTGTTAGAATTCGATATGTTCTGCCAAGGGTT |
Xho Tet forward | CCGGCTCGAGGGTAGCTCAGAGAACCTTCG |
Xho Tet reverse | CCGGCTCGAGGATATGTTCTGCCAAGGGTT |