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Figure 4 | BMC Microbiology

Figure 4

From: Environmental stresses inhibit splicing in the aquatic fungus Blastocladiella emersonii

Figure 4

Effect of thermotolerance on hsp70 mRNA splicing inhibition. A -- Nuclease S1 protection assays were performed using a 5' end-labeled probe (the same used in Figure 3) and 50 μg of total RNA isolated from cells incubated at the following temperatures for 30 min: 27°C and 38°C (lane 1); 27°C and 42°C (lane 2); 27°C, 38°C, 27°C and 42°C (lane 3); 27°C, 38°C, 27°C, 42°C and 27°C (lane 4). B -- Cells incubated at 27°C for 30 min and then with 250 μM CdCl2 for 60 min (lane 1); cells incubated at 27°C for 30 min, at 38°C for 30 min, at 27°C for 30 min, and then with 250 μM CdCl2 for 60 min (lane 2); cells incubated at 27°C for 30 min, with 250 μM CdCl2 for 60 min and then at 27°C for 60 min (lane 3); cells incubated at 27°C for 30 min, at 38°C for 30 min, at 27°C for 30 min, with 250 μM CdCl2 for 60 min and then at 27°C for 60 min (lane 4).

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