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Table 7 Summary of the performance of the biochemical, chromogenic and PCR methods for Cronobacter spp. identity confirmation.

From: Isolation of Cronobacter spp. (formerly Enterobacter sakazakii) from infant food, herbs and environmental samples and the subsequent identification and confirmation of the isolates using biochemical, chromogenic assays, PCR and 16S rRNA sequencing

Test Method

Number of confirmed isolates

Number of false positives

Number of false negatives

API 20E

42

13

-

α-MUG

34

5

-

DFI

32

3

-

EsPM

33

7

3

Glu A PCR primers

26

3

6

Glu B*PCR primers

3

1

3

SG PCR primers

36

10

2

SI PCR primers

36

10

3

Ska PCR primers

25

-

4

OmpA PCR primers

27

-

2

Zpx 94 bp PCR primers

32

13

10

Zpx 350 bp PCR primers

32

6

3

  1. *: Only 9 samples were tested using these primers