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Table 5 Composition of the 16 pools of MSP-1 derived peptides.

From: Population diversity and antibody selective pressure to Plasmodium falciparum MSP1 block2 locus in an African malaria-endemic setting

Pool

Localisation

Peptide sequence

P1

Block 1 Mad20

01 TGYSLFQKEKMVLNE

60 TGYGLFQKEKMVLNE

45 TGYSLFHKEKMILNE

45 TGYSLFHKEKMILNE

73 TGYGLFHKEKMLLNE

 

P2

Block 3

10 RTNPSDNSSDSDAKS

27 RTNPSDNSSDSNTKT

28 SSDSNTKTYADLKHR

40 GAANPSDDSSDSDAK

72 DASDSDAKSYADLKH

 

P3

N-term MAD20

02 KEKMVLNEGTSGTAV

03 EGTSGTAVTTSTPGS

13 VTTSTPGSKGSVASS

04 VTTSTPGSGGSVTSG

29 VTTSTPGSSGSVASG

 

P4

N-term MAD20

11 VTTSTPGSSGSVTSG

25 VTTSTPGSKGSVTSG

14 SKGSVASSGSVASGG

05 SGGSVTSGGSGGSVA

  

P5

Central MAD20

16 SGGSVTSGGSVTSGG

17 GGSVTSGGSGASVAS

26 SSGSVTSGGSVASVA

30 SSGSVASGGSVASVA

22 GSVTSVASVASVASV

 

P6

Central MAD20

15 GGSGGSVASGGSVAS

18 GSGASVASVASVASV

32 SVASGGSVASGGSGN

23 SVASVASVASVASGG

07 ASVASGGSGGSVASG

 

P7

C-term MAD20

06 GGSGGSVASVASGGS

31 GGSVASVASGGSGGS

19 SVASVASVASGGSGN

08 SGGSVASGGSGNSRR

12 ASVASGASGGSGNSR

 

P8

C-term MAD20

24 VASVASGGSGNSRRT

20 VASGGSGNSRRTNPS

09 GGSGNSRRTNPSDNS

21 NSRRTNPSDNSSDSD

  

P9

N-term RO33

33 KEKMVLKDGANTQVV

34 DGANTQVVAKPADAV

41 DGANTQVVAKPVPAV

43 DGANTQVVAKPAGAV

35 VAKPADAVSTQSAKN

 

P10

C-term RO33

42 VAKPVPAVSTQSAKN

44 VAKPAGAVSTQSAKN

36 VSTQSAKNPPGATVP

37 NPPGATVPSGTASTK

38 PSGTASTKGAIRSPG

39 KGAIRSPGAANPSDD

P11

N-term K1

46 KEKMILNEEEITTKG

61 KEKMVLNEEEITTKG

74 KEKMLLNEEEITTKG

47 EEEITTKGASAQSGT

76 EEEITTKGASAQGSS

 

P12

N-term K1

48 GASAQSGTSGTSGTS

62 GASAQSGASAQSGAS

77 GASAQGSSGPSGTPS

56 TSGTSGTSGTSGTSG

49 TSGTSGTSGPSGPSG

 

P13

Central K1

67 TSGTSGPSGPSGTSP

75 TSGTSGPSGTSPSSR

57 GTSGTSAQSGTSGTS

65 GTSAPSGSGTSPSSR

80 GTSGPSGTGPSGTSP

 

P14

Central K1

79 SGTSGPSGTSGPSGT

50 SGPSGPSGTSPSSRS

68 SGPSGTSPSSRSNTL

78 SGPSGTPSGTSGPSG

58 SAQSGTSGTSAQSGT

64 SAQSGPSGTSAPSGS

P15

C-term K1

63 QSGASATSAQSGPSG

59 TSGTSGTSGTSPSSR

51 GTSPSSRSNTLPRSN

69 PSSRSNTLPRSNTSS

52 SNTLPRSNTSSGASP

81 LPRSNTSSGASPPAD

P16

C-term K1

70 LPRSNTSSGAIPPAD

66 SNTSSGAPPADASDS

53 NTSSGASPPADASDS

71 SGAIPPADASDSDAK

82 SGASPPADASDSDAK

54 PPADASDSDAKSYAD

  1. The 15-mer peptide sequence is represented in single letter code, and the location of the peptide in the region is indicated. Pools contained equimolar amounts of four to six biotinylated peptides (0.1 nM each).
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BMC Microbiology

ISSN: 1471-2180

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