Skip to main content

Table 1 Bacterial strains and plasmids.

From: Environmental and intracellular regulation of Francisella tularensis ripA

Strains or Plasmid

Description

Source or Reference

Bacteria

  

Francisella tularensis LVS

F. tularensis live vaccine strain

CDC, Atlanta, GA

   ripA::Tn5

Tn5 ripA transposon mutant

[21]

   ripA::pBSK Φ(ripA'-lacZ)1

Plasmid cointegrate

This work

   ripA:: pBSK Φ(ripA'-lacZ)2

Plasmid cointegrate

This work

   iglA:: pBSK Φ(iglA'-lacZ)

Plasmid cointegrate

This work

   Φ(ripA'-TC)

Exchanged allele

This work

   ΔmglA

Inframe deletion of mglA

This work

   ΔmglA ripA:: pBSK Φ(ripA'-lacZ)2

Plasmid cointegrate

This work

   ΔmglA iglA:: pBSK Φ(iglA'-lacZ)

Plasmid cointegrate

This work

   ΔsspA

Inframe deletion of sspA

This work

   ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2

Plasmid cointegrate

This work

   ΔsspA iglA:: pBSK Φ(iglA'-lacZ)

Plasmid cointegrate

This work

   ΔmglA ΔsspA

Inframe gene deletions

This work

   ΔmglA ΔsspA ripA:: pBSK Φ(ripA'-lacZ)2

Plasmid cointegrate

This work

   ΔmglA ΔsspA iglA:: pBSK Φ(iglA'-lacZ)

Plasmid cointegrate

This work

Plasmids

  

   pBSK bla lacZ

pBluescript cloning vector

Stratagene

   pBSK lacZ aphA1 bla

Transcriptional lacZ fusion

This work

   pBSK lacZ cat bla

Translational lacZ fusion

This work

   pBSK Φ(ripA'-lacZ)2 aphA1 bla

Francisella suicide vector

This work

   pBSK Φ(ripA'-lacZ)1 cat

Francisella suicide vector

This work

   pBSK Φ(iglA'-lacZ)2 aphA1

Francisella suicide vector

This work

   pMP590

Francisella sacB suicide vector

[47]

   pMP590 ΔmglA

mglA allelic exchange vector

This work

   pMP590 ΔsspA

sspA allelic exchange vector

This work

   pMP590 Φ(ripA'-TC)

Φ(ripA'-TC) suicide vector

This work

   pMP633

Francisella shuttle vector

[47]

   pMP633 mglA+

mglA+ with native promoter

This work

   pMP633 sspA+

sspA+ with native promoter

This work

   pKK MCS

Francisella shuttle vector

[21]

   pKK MCS Φ(ripA'-lacZ)1

translational fusion

This work

   pKK MCS Φ(ripA'-lacZ)1a

-10 mutation

This work

   pKK MCS Φ(ripA'-lacZ)1b

RBS mutation

This work

   pKK MCS Φ(ripA'-lacZ)1c

lacZ frameshift

This work

   pKK MCS Φ(ripA'-lacZ)1d

ripA core promoter

This work

   pKK MCS Φ(ripA'-lacZ)2

transcriptional fusion

This work

   pKK MCS Φ(ripA'-TC)

ripA-CT tetracysteine tag fusion

This work