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Figure 6 | BMC Microbiology

Figure 6

From: Environmental and intracellular regulation of Francisella tularensis ripA

Figure 6

Analysis of the effects of pH on expression. Effect of pH on F. tularensis LVS ripA expression. All experiments were performed using mid exponential phase bacteria cultured in Chamberlains defined media at pH 5.5 or pH 7.5. Data are presented as mean values with error bars representing one standard deviation. (a) β-galactosidase activity of F. tularensis LVS pKK ripA'-lacZ1 at pH 5.5 and pH 7.5. Difference in expression levels were significant (P < 0.01). (b) β-galactosidase activity of F. tularensis LVS ripA'-lacZ2 at pH 5.5 and pH 7.5. Difference in expression levels were significant (P < 0.01). (c) F. tularensis LVS ripA RNA concentrations displayed as tul4 normalized mean trace (Int mm) on four independent RT-PCR reactions using purified total RNA samples of mid exponential F. tularensis LVS cultured at pH 5.5 and pH 7.5. Difference in expression levels were significant (P < 0.01). (d) RipA-TC concentration in whole cell lysates of mid exponential phase F. tularensis LVS ripA'-TC cultured at pH 5.5 and pH 7.5. Concentrations were measured using densitometry of the specific in-gel fluorescence of FlAsH™ labeled RipA-TC. Four independent samples were used to calculate mean expression. Difference in expression was significant (P < 0.01).

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