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Figure 2 | BMC Microbiology

Figure 2

From: Environmental and intracellular regulation of Francisella tularensis ripA

Figure 2

The ripA'-lacZ reporter sequence and expression. (a) Multiple sequence alignment of translational and transcriptional ripA'-lacZ fusions. Predicted -10 and RBS sequences are boxed with introduced mutations in each highlighted. (b) β-galactosidase activity of chromosomal and plasmid translational and transcriptional F. tularensis LVS ripA'-lacZ reporter strains displayed as mean Miller units. Error bars represent the standard deviation of three samples. (c) β-galactosidase activity of F. tularensis LVS plasmid translational ripA'-lacZ1 promoter mutations displayed as mean Miller units. Error bars represent the standard deviation of three samples.

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