Figure 4
From: Inactivation of staphylococcal virulence factors using a light-activated antimicrobial agent
The effect of 20 μM methylene blue and different laser light doses on the proteolytic activity of V8 protease. V8 protease was either kept in the dark (L-) or irradiated with laser light doses of 1.93 J/cm2, 3.86 J/cm2 and 9.65 J/cm2 (L+) in the presence of an equal volume of either PBS (S-) (white bars) or 20 μM methylene blue (S+) (black bars). Following irradiation, the activity of the enzyme was assessed using the azocasein hydrolysis assay. Error bars represent the standard deviation from the mean. *** P < 0.001 (ANOVA). Experiments were performed three times in triplicate and the combined data are shown.