Figure 2From: Cytokinesis is blocked in mammalian cells transfected with Chlamydia trachomatis gene CT223Expression of CT223 at different times post infection and differential reactivity with specific antibodies. DNA in all panels is labeled with DAPI (blue) and the bar in panel F represents 10 microns in each image. Cells were infected at an MOI of approximately 0.2 and fixed with 100% methanol prior to antibody labeling. Panels A-D: Fluorescent microscopy of McCoy cells infected with either strain LGV-434 (A, B) or J/UW36 (C, D). Cells were fixed at different times p.i. (A: 12 h, C: 18 h, B, D: 38 h). In panels A-D, cells were labeled with monoclonal anti-CT223p antibody (green) and anti-HSP60 (red). Note that labeling of CT223p is patchy in each strain at the early times points p.i. (A, C) but the labeling is distinct between strains at 38 h p.i. (B, D). Panels E-H: Cells were infected with strain J/UW36 (E, F) or J(s)1980 (G, H) and fixed 30 hours p.i. Cells were then labeled with either polyclonal anti-CT223p antisera (E, G) or monoclonal anti-CT223p antibody (F, H), both of which are labeled red. Note that CT223p is labeled by the polyclonal antisera in each strain, while the monoclonal anti-CT223p does not label the protein in strain J(s)1980.Back to article page