Strain or plasmid | Description | Reference |
---|---|---|
Arthrobacter | Â | Â |
   FB24 | CrR | [6] |
   D11 | CrS derivative of FB24 | This work |
E. coli | Â | Â |
   JM110 | dam - dcm - | Stratagene |
Plasmids | Â | Â |
pAOWA10128 | 7.3 kb insert in pMCL200 obtained from DOE-JGI. Contains Arth_4248-Arth_4254. | DOE-JGI |
pBluescript II SK+ | 3.0 kb, ApR, lacZ, used for sublconing inserts prior to ligation into pART2. | Promega |
pART2 | 4.6 kb, KmR, pCG100 ori, ColE1 ori, vector for expression in Arthrobacter | [55] |
pKH11 | 10.6 kb PCR product from FB24 plasmid 3 (CP000457) containing Arth_4247-4255 in pBluescript II SK+ | This worka |
pKH12 | Insert from pKH11 cloned into pART2 | This work |
pKH21 | 7.3 kb insert from pAOWA10128 in pBluescript II SK+ | This work |
pKH22 | Insert from pKH21 cloned into pART2 | This work |
pKH32 | 3.7 kb EcoRI-KpnI fragment from pKH21 cloned into pART2. Contains Arth_4248-4249. | This work |
pKH42 | 3.8 kb XhoI-BglII fragment from pKH21 cloned into pART2. Contains Arth_4251-Arth_4254. | This work |
pKH52 | 8.3 kb insert from MluI-BglII digest of pKH11 to delete Arth_4252 and Arth_4252 cloned into pART2 | This work |
pKH62 | pKH22 digested with SfiI to delete Arth_4249-Arth_4252. | This work |
pKH72 | pKH12 digested with ScaI and XbaI to delete Arth_4247. | This work |