Table 2 Bacterial strains and plasmids
From: 2D proteome analysis initiates new Insights on the SalmonellaTyphimurium LuxS protein
Name | Description | Reference |
|---|---|---|
S. Typhimurium SL1344 | Parent strain | [44] |
CMPG5649 | S. Typhimurium luxS3xFLAG | This work |
CMPG5702 | Insertion of a kanamycin resistance gene in the ClaI restriction site of the luxS gene of SL1344;luxS::KmR | This work |
CMPG5726 | S. Typhimurium SL1344 ΔphoN | This work |
CMPG5729 | S. Typhimurium SL1344 luxSβla | This work |
pTn5-blam | Plasmid used as template for construction of CMPG5729 | [49] |
pKD4 | Plasmid used as template for construction of CMPG5726 | [48] |
pSUB11 | Plasmid used as template for construction of CMPG5649 | [50] |
pCVD442 | Positive selection suicide vector | [52] |
pACYC184 | Derivative of p15A; TcR; CmR | [54] |
pFAJ1708 | Derivative of RK-2; ApR; TcR; contains nptII promoter of pUC18-2 | [55] |
pCMPG5664 | pACYC184 containing the luxS gene of SL1344 | [13] |
pCMPG5700 | pUCBM20 containing the luxS gene and flanking regions of SL1344 in SacI restriction site; ApR, lacZ | This work |
pCMPG5718 | pACYC184 with luxS: Y88→F88 | This work |
pCMPG5719 | pACYC184 with luxS: Y88→D88 | This work |
pCMPG5720 | pACYC184 with luxS: Y126→F126 | This work |
pCMPG5721 | pACYC184 with luxS: Y126→D126 | This work |
pCMPG5722 | pACYC184 with luxS: Y131→D131 | This work |
pCMPG5723 | pACYC184 with luxS: Y131→F131 | This work |
pCMPG5730 | pACYC184 vector containing luxSphoA fusion protein with luxS promoter | This work |
pCMPG5734 | pFAJ1708 containing PhoA with signal peptide; positive control | This work |
pCMPG5748 | pFAJ1708 containing PhoA without signal peptide; negative control | This work |
pCMPG5743 | pCMPG5664 with point mutation in luxS: C83→A83 | This work |