malT knockout mutation invokes a stringent type gene-expression profile in Actinobacillus pleuropneumoniae in bronchoalveolar fluid

Table 5 Verification of microarray data by real-time PCR

Gene	Putative function	ΔΔC_T ± SD	Fold change by real-time PCR	Fold change by microarray¹
dmsA (T)	Anaerobic dimethyl sulfoxide reductase chain A precursor	3.45 ± 1.41	0.091 (0.03-0.24)	0.15
dmsA (R)		0 ± 0.51	1 (0.69-1.42)
dmsB (T)	Anaerobic dimethyl sulfoxide reductase chain B	2.54 ± 1.61	0.17 (0.05-0.52)	0.34
dmsB (R)		0 ± 0.46	1 (0.72-1.38)
napB (T)	Nitrate reductase cytochrome c-type subunit	2.24 ± 0.41	0.21 (0.15-0.28)	0.17
napB (R)		0 ± 0.49	1 (0.71-1.40)
napF (T)	Ferredoxin-type protein NapF	2.24 ± 0.46	0.21 (0.07-0.61)	0.09
napF (R)		0 ± 0.47	1 (0.71-1.39)
napD (T)	Putative napD protein	2.39 ± 0.34	0.18 (0.14-0.24)	0.18
napD (R)		0 ± 0.54	1 (0.68-1.46)
ilvH (T)	Acetolactate synthase small subunit	-2.60 ± 0.36	6.08 (4.68-7.90)	6.14
ilvH (R)		0 ± 0.45	1 (0.70-1.41)
pgaA (T)	Biofilm PGA synthesis protein PgaA precursor	-2.04 ± 1.08	4.11 (1.94-8.70)	8.18
pgaA (R)		0 ± 0.74	1 (0.59-1.67)
pgaC (T)	Biofilm PGA synthesis N-glycosyltransferase PgaC	-2.47 ± 0.42	5.54 (4.12-7.45)	6.23
pgaC (R)		0 ± 1.05	1(0.48-1.07)
apxIVA (T)	RTX toxin protein	-3.01 ± 1.12	8.06 (3.69-17.61)	6.5
apxIVA (R)		0 ± 0.60	1 (0.65-1.52)
relA (T)	GTP pyrophosphokinase	-0.95 ± 0.42	2.0 (1.44-2.56)	6.30
relA (R)		0 ± 0.59	1(0.66-1.51)
lamB (T)²	Maltoporin	1.03 ± 0.39	0.49 (0.37-0.64)	na³
lamB (R)		0 ± 0.23	1 (0.85-1.17)

¹Fold change is the fold increase or decrease in the level of expression of a gene in the malT mutant (target sample, abbreviated as T) relative to the level of expression of the gene in the wild type (calibrator or reference sample, abbreviated as R) in BALF except for the lamB gene² whose expression was compared in BHI to examine the effect of the malT knockout mutation on the expression of the lamB gene. ³ Not applicable.
Values in the parentheses represent the range in the fold change.

ISSN: 1471-2180