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Table 2 List of primers used for the various PCR reactions

From: Molecular features of heterogeneous vancomycin-intermediate Staphylococcus aureus strains isolated from bacteremic patients

Locus/Type

Primer

Nucleotide Sequence

Size (bp)

SCC mec

A

CIF2 F2

TTCGAGTTGCTGATGAAGAAGG

495

 

CIF2 R2

ATTTACCACAAGGACTACCAGC

 

B

KDP F1

AATCATCTGCCATTGGTGATGC

284

 

KDP R1

CGAATGAAGTGAAAGAAAGTGG

 

C

MECI P2

ATCAAGACTTGCATTCAGGC

209

 

MECI P3

GCGGTTTCAATTCACTTGTC

 

D

DCS F2

CATCCTATGATAGCTTGGTC

342

 

DCS R1

CTAAATCATAGCCATGACCG

 

E

RIF4 F3

GTGATTGTTCGAGATATGTGG

243

 

RIF4 R9

CGCTTTATCTGTATCTATCGC

 

F

RIF5 F10

TTCTTAAGTACACGCTGAATCG

414

 

RIF5 R13

GTCACAGTAATTCCATCAATGC

 

G

IS431 P4

CAGGTCTCTTCAGATCTACG

381

 

pUB110 R1

GAGCCATAAACACCAATAGCC

 

H

IS431 P4

CAGGTCTCTTCAGATCTACG

303

 

pT181 R1

GAAGAATGGGGAAAGCTTCAC

 

mecA

MECA P4

TCCAGATTACAACTTCACCAGG

162

 

MECA P7

CCACTTCATATCTTGTAACG

 

SCC mec type V

Type I

Type I-F

GCTTTAAAGAGTGTCGTTACAGG

613

 

Type I-R

GTTCTCTCATAGTATGACGTCC

 

Type II

Type II-F

CGTTGAAGATGATGAAGCG

398

 

Type II-R

CGAAATCAATGGTTAATGGACC

 

Type III

Type III-F

CCATATTGTGTACGATGCG

280

 

Type III-R

CCTTAGTTGTCGTAACAGATCG

 

Type IVa

Type IVa-F

GCCTTATTCGAAGAAACCG

776

 

Type IVa-R

CTACTCTTCTGAAAAGCGTCG

 

Type IVb

Type IVb-F

TCTGGAATTACTTCAGCTGC

493

 

Type IVb-R

AAACAATATTGCTCTCCCTC

 

Type IVc

Type IVc-F

ACAATATTTGTATTATCGGAGAGC

200

 

Type IVc-R

TTGGTATGAGGTATTGCTGG

 

Type IVd

Type IVd-F5

CTCAAAATACGGACCCCAATACA

881

 

Type IVd-R6

TGCTCCAGTAATTGCTAAAG

 

Type V

Type V-F

GAACATTGTTACTTAAATGAGCG

325

 

Type V-R

TGAAAGTTGTACCCTTGACACC

 

mecA

MecA147-F

GTG AAG ATA TACCAAGTG ATT

147

 

MecA147-R

ATG CGCTATAGATTG AAAGGAT

 

Panton-Valentine leukocidin (PVL)

 

luk-PV-1

ATCATTAGGTAAAATGTCTGGACATGATCCA

433

 

luk-PV-2

GCATCAASTGTATTGGATAGCAAAAGC

 

Accessory gene regulator ( agr )

 

agrSa agr1-4Sa-1

ATGCACATGG TGCACATGC

 
 

agr-1Sa agr1Sa-2

GTCACAAGTA CTATAAGCTG CGAT

439

 

agr-2Sa agr2Sa-2

TATTACTAAT TGAAAAGTGC CATAGC

572

 

agr-3Sa agr3Sa-2

GTAATGTAAT AGCTTGTATA ATAATACCCAG

321

 

agr-4Sa agr4Sa-2

CGATAATGCC GTAATACCCG

657

Gyrase

 

gyrA-F

AGTACATCGT CGTATACTAT ATGG

280

 

gyrA-R

ATCACGTAAC AGTTCAAGTGTG

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