Extracellular secretion of Carocin S1 in Pectobacterium carotovorum subsp. carotovorumoccurs via the type III secretion system integral to the bacterial flagellum

Table 1 Bacteria and plasmids used in this study

Bacterium or plasmid	Relevant characteristics	Source
E. coli
1830	pro^-met^-Km^rNm^r, containing transposon Tn5 on the ?sucidal? plasmid pJB4JI	Gantotti et al. [37]
DH5	supE44hsdR17recA1endA1gyrA1thi-1relA1	Hanahan and Reusch et al [26, 38]
Pectobacterium carotovorum subsp. carotovorum
89-H-4	putative biocontrol agent	Laboratory stock
H-rif-8-6	89-H-4, Rif^r	this work
Ea1068	wild type	Laboratory stock
T-29	wild type	Laboratory stock
E108	wild type	Laboratory stock
A-100	wild type	Laboratory stock
86-H-2	wild type	Laboratory stock
TH12-2	H-rif-8-2, flhC:: Tn5, Rif^r, Kan^r	this work
KH17	H-rif-8-2, flh D::Kan, Rif^r, Kan^r	this work
FliC-KO	H-rif-8-2, fli C::Kam, Rif^r, Kam^r	this work
FlhA-KO	H-rif-8-2, flh A::Kam, Rif^r, Kam^r	this work
plasmid
pBR322	Amp^r, Kan^r	Bolivar et al [39]
pBYL2DC	Amp^r, flhDC	this work
pBYL2C	Amp^r, flhC	this work
pBYL2D	Amp^r, flhD	this work
pBFC	Amp^r, fliC	this work
pBFA	Amp^r, flhA	this work

Amp^r indicates ampcillin resistance, Rif^r indicates rifampicin resistance, and Kan^r indicates Kanamycin resistance.

ISSN: 1471-2180