Table 1 Oligonucleotide primers and fluorescent probes used in RT-PCR
From: Involvement of SPI-2-encoded SpiC in flagellum synthesis in Salmonella enterica serovar Typhimurium
Target gene | Nucleotide sequence (5'-3') |
|---|---|
fliD | CGCGAAGCTGAACGTAAACG |
| Â | GCGTCGGTTACGGTATTGC |
| Â | 6-FAM-TCTGACGCTCAATGTC |
motA | GCTTTCATGGGTTTCAATCTCTTCA |
| Â | CAGCGGCAACATGAATACGTT |
| Â | 6-FAM-TCAACGCTTCAATTTC |
fliA | TCGATGCTATCGCCATGCT |
| Â | TTGCGGAGTATCGTCAGATGTT |
| Â | 6-FAM-CGCCACTCATCGTAAGA |
fliF | GCAGACGGAAGAGCACTACAG |
| Â | GCCTACCTGTTCGCTAATATTCAAC |
| Â | 6-FAM-TCGAAGGCCACTCTGC |
flgB | CGTCGCGTTAACGTTGACTTC |
| Â | TCCACTGCGGGAGAAGAGA |
| Â | 6-FAM-CTCTCACCATATTCCC |
flhD | TGATGATCGTCAAACCGGAAA |
| Â | TGCCGCAGATGGTCAAACTG |
| Â | 6-FAM-AACTAACTGGTTCGTCTCC |
16S rRNA | AGATGGGATTAGCTTGTTGGTGA |
| Â | GTAACGTCAATGCTGCGGTTA |
| Â | 6-FAM-CCACAACACCTTCCTC |