Table 1 Bacterial strains and plasmids

S. enterica serovar Typhimurium

14028

Wild type

ATCC 14028

14028Δzwf

Δzwf82

Replacement of zwf gene with Δzwf82 by homologous recombination

14028 gnd

gnd-189::MudJ (Kan^R)

P22·DM4483 × 14028 → Kan₄₀^R

YS1646(=VNP20009)

ΔmsbB2 ΔpurI ΔSuwwan

[9, 31]

VNP20057

ΔmsbB2 ΔpurI zwf81::Tn5 (Kan^R) ΔSuwwan

YS1646 × P22 Tn5 pool (on 14028) → selection on LB plates in 5% CO₂

YS1

msbB1::Ωtet

[4]

YS1 msbB⁺

msbB1::Ωtet/pSM21msbB⁺ (Amp^R)

Plasmid pSM21 [4] into YS1

YS1 zwf(=YS11)

msbB1::Ωtet zwf:Tn5 (Kan^R)

P22·VNP20057 × YS1 → Kan₂₀^R

YS873

msbB1::Ωtet somA1 zbj10:Tn10

[4]

YS873 msbB⁺ (=YS8731)

msbB1::Ωtet somA1 zbj10:Tn10/pSM21msbB⁺ (Amp^R)

Plasmid pSM21 [4] into YS873

YS873 zwf(=YS8732)

msbB1::Ωtet somA1 zbj10:Tn10 zwf81:Tn5 (Kan^R)

P22·VNP20057 × YS873 → Kan₂₀^R

YS873 Δzwf (=YS8733)

msbB1::Ωtet somA1 zbj10:Tn10 Δzwf82

Replacement of zwf81::Tn5 gene in YS873zwf with Δzwf82 by homologous recombination

YS873 gnd (=YS8734)

msbB1::Ωtet somA1 zbj10:Tn10 gnd-189::MudJ (Kan^R)

P22·DM4483 × YS873 → Kan₁₀^R

LT2

Wild type

ATCC 15277

DM4483

gnd-189::MudJ (Kan^R)

Gift of Diana Downs and Eugene I. Vivas, U. of Wisconsin

YS501

recD541::Tn10 dCm hsdSA29 hsdSB121 hsdL6 metA22 metE551 trpC2 ilv-452 H1-b H2-e,n,x fla-66 nml(-) rpsL120 xyl-404 galE719

[5]

Salmonella enterica serovar Typhi CS029

Salmonella enterica serovar Typhi ATCC 33458

E. coli K-12 MG1655

F- l- rph-1

[32]

KL423

F- l- rph-1 msbB1:: ΩCm

[4]

pCVD442

Amp^R

[10]

pCVD442Δzwf 82

Amp^R

This study

pSP72

Amp^R

Promega Corporation

pSP72lacZ

lacZ, Amp^R

This study

pSM21

msbB, Amp^R

[4]