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Figure 8 | BMC Microbiology

Figure 8

From: msbB deletion confers acute sensitivity to CO2 in Salmonella enterica serovar Typhimurium that can be suppressed by a loss-of-function mutation in zwf

Figure 8

β-galactosidase release assays confirm cell lysis in LB broth, pH 6.6, in air; zwf inhibits cell lysis in LB broth, pH 6.6, in air and in LB broth, pH 6.6, but not pH 7.6, in the presence of 5% CO 2 . Release of β-galactosidase from the cytosol of the bacteria was used to test if the growth defects observed in YS873 and YS873 zwf resulted from cell lysis. Strains grown in LB broth at either pH 6.5, or pH 7.5, under either ambient air (A) or 5% CO2 (B) conditions.

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