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Table 2 MIC results and Erg11p substitutions for 25 C. albicans isolates with reduced susceptibility to fluconazole and 23 fluconazole-susceptible isolates by RCAa and ERG11 sequencing.

From: Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing

  

MIC (μg/ml)

Erg 11p amino acid substitutions

    

D

D

E

F

F

G

G

G

G

K

K

R

S

V

V

Y

    

1

2

2

1

4

3

4

4

4

1

1

4

4

4

4

1

Patient/isolate no.

Site

FLU a

VOR a

1

7

6

4

4

0

4

6

6

2

4

6

0

3

8

3

    

6

8

6

5

9

7

8

4

5

8

3

7

5

7

8

2

    

E

E

D

L

S

S

E

S

S

T

R

K

F

I

I

H

Isolates with reduced fluconazole susceptibility

1b

Oropharynx

16

0.25

  

+

+

          

+

 

2b

Vagina

>256

0.03

                

3-Ab, c

Oropharynx

16

0.25

  

+

+

          

+

 

- Bb, c

Oropharynx

16

0.5

  

+

+

          

+

 

4d

Oropharynx

256

0.25

  

+

       

+

   

+

 

5d

Oropharynx

256

0.125

     

+

          

6-Ac, d

Oropharynx

256

>16

  

+

             

-Bc, d

Oropharynx

256

>16

+

               

7d

Oropharynx

256

>16

  

+

    

+

  

+

   

+

 

8-Ac, d

Oropharynx

256

0.5

  

+

 

+

         

+

 

-Bc, d

Oropharynx

256

1

  

+

 

+

         

+

 

9d

Oropharynx

256

>16

  

+

          

+

  

10d

Oropharynx

256

2

  

+

         

+

 

+

+

11d

Oropharynx

256

0.008

  

+

          

+

  

12-Ac, d

Oropharynx

256

2

       

+

        

-Bc, d

Sputum

32

0.5

+

      

+

+

       

13d

Vagina

32

0.016

         

+

      

14d

Blood

256

>16

+

        

+

      

15d

Bile

256

16

             

+

  

16-Ac, d

Oropharynx

16

0.125

+

     

+

  

+

      

-Bc

Oropharynx

256

2

+

    

+

+

  

+

      

-Cc, d

Oropharynx

256

16

+

    

+

+

+

 

+

      

17d

Oropharynx

64

0.5

 

+

              

18d

Oropharynx

128

2

+

        

+

 

+

    

19d

Oropharynx

256

0.5

+

        

+

+

     

Fluconazole-susceptible isolates b

ATCC 10231

UNa

0.125

0.008

                

ATCC 90028

Blood

025

0.03

                

20

Blood

0.25

<0.008

  

+

          

+

  

21

Oropharynx

0.12

0.008

+

 

+

             

22

Blood

0.5

0.016

                

23

Blood

0.5

0.016

                

24

Blood

0.25

0.008

                

25

Blood

0.25

0.008

  

+

           

+

 

26

Blood

0.5

0.008

+

 

+

             

27

Blood

0.5

0.008

+

 

+

             

28

Blood

0.25

<0.008

  

+

          

+

  

29

Skin

1

0.016

+

        

+

      

30

Peritoneal fluid

1

2

+

 

+

             

31

Blood

0.12

0.125

+

 

+

           

+

 

32

Blood

0.125

0.008

+

        

+

      

33

Blood

0.5

0.008

+

 

+

           

+

 

34

Tissue

0.25

0.008

+

        

+

      

35

Blood

2

0.008

  

+

           

+

 

36

Blood

0.25

0.016

  

+

           

+

 

37

Liver

0.5

<0.008

  

+

           

+

 

38

Blood

0.25

<0.008

+

 

+

           

+

 

39

Blood

0.125

<0.008

  

+

             

40

Bone

0.25

<0.008

+

 

+

             
  1. The "+" sign denotes the presence of the mutation.
  2. aAbbreviations: RCA, rolling circle amplification; FLU, fluconazole; VOR, voriconazole; UN, unknown.
  3. b Isolates from the Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney.
  4. c The "A" and "B" notation of patient numbers refers to isolates cultured sequentially from the same patient at different times.
  5. dIsolates obtained from the Mycology Unit, Women's and Children's Hospital, Adelaide.