Figure 5

Flow cytometry and SDS-PAGE analysis of S. gordonii surface enolase. A)- Intact S. gordonii preparation was stained with a polyclonal antibody for α-enolase (C-19). Specific secondary antibody coupled with Texas Red (anti goat) was used for detection (filled black) and compared with isotype control (filled gray). Results are shown as one representative experiment of three different S. gordonii preparations. B)- An aliquot from the surface extract from S. gordonii were separated on a 4–20% gradient SDS-PAGE gel, unreduced (U, lane 1) and reduced (R, lane 2). The gel was Western blotted onto nitrocellulose membrane and probed with anti-enolase antibody. Positions of the molecular markers are indicated (kDa).