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Table 3 Plasmids used in this study

From: Cj0596 is a periplasmic peptidyl prolyl cis-trans isomerase involved in Campylobacter jejuni motility, invasion, and colonization

Plasmid

Description

Source

pET-20b(+)

Cloning vector, AmpR

Novagen

pCR II-TOPO

Cloning vector, KanR AmpR

Invitrogen

pKR001

cj0596 gene and flanking region in pCR II-TOPO

This study

pKR002

Self-ligated inverse PCR product of pKR001 with cj0596 removed

This study

pKR016

cj0596 gene lacking signal sequence and stop codon in pCR II-TOPO

This study

pKR017

cj0596 gene lacking signal sequence and stop codon in pET-20b(+)

This study

pKR018

cat cassette in pCR II-TOPO

This study

pKR019

H. pylori 84–183 rpsL gene in pCR II-TOPO

This study

pKR020

cat cassette in pKR002

This study

pKR021

rpsL HP /cat construct in pKR002

This study