Table 1 Primers used to amplify AvBD and chicken beta-actin genes

Actin-F

Actin-R

5'-tgcgtgacatcaaggagaag-3'

5'-gaccatcagggagttcatagc-3'

111 bp

AvBD-1-F

AvBD-1-R

5'-cgaaagagtggcttctgtgc-3'

5'-ggtgatgtcctgcttggg-3'

156 bp

AvBD-2-F

AvBD-2-R

5'-aggtttctccagggttgt-3'

5'-tgcattccaaggccattt-3'

146 bp

AvBD-3-F

AvBD-3-R

5'-ccactcagtgcagaataagag-3'

5'-aattcagggcatcaacctc-3'

131 bp

AvBD-4-F

AvBD-4-R

5'-catctcagtgtcgtttctctgc-3'

5'-cgcgatatccacattgcatg-3'

157 bp

AvBD-5-F

AvBD-5-R

5'-ctgccagcaagaaaggaacctg-3'

5'-gtaatcctcgagcaagggaca-3'

155 bp

AvBD-6-F

AvBD-6-R

5'-aggatttcacatcccagccgtg-3'

5'-cgacatggcccaggaatgcag-3'

156 bp

AvBD-7-F

AvBD-7-R

5'-tggagaagggagacagaaggc a-3'

5'-cgaagcctacaagtatcaat-3'

177 bp

AvBD-8-F

AvBD-8-R

5'-acagtgtgagcaggcaggaggga-3'

5'-gaagagctgcttagctggtct-3'

153 bp

AvBD-9-F

AvBD-9-R

5'-gcaaaggctattccacagcag-3'

5'-ggagcacggcatgcaacaa-3'

167 bp

AvBD-10-F

AvBD-10-R

5'-tggggcacgcagtccacaac-3'

5'-catgccccagcacggcagaa-3'

157 bp

AvBD-11-F

AvBD-11-R

5'-actgcatccgttccaaagtctg-3'

5'-gtcccagctgttcttccag-3'

168 bp

AvBD-12-F

AvBD-12-R

5'-cccagcaggaccaaagcaatg-3'

5'-agtacttagccaggtattcc-3

157 bp

AvBD-13-F

AvBD-13-R

5'-catcgttgtcattctcctcctc-3'

5'-ggtggagaacctgcagcagcg-3'

163 bp

AvBD-14-F

AvBD-14-R

5'-atgggcatattcctcctgt-3'

5'-cactttgccagtccattg t-3'

161 bp