Photonic plasmid stability of transformed Salmonella Typhimurium: A comparison of three unique plasmids

BMC Microbiology

Table 3 Detection limits of luminescent Salmonella typhimurium.

Item	Bacterial concentration (CFU)	Photonic emissions (RLU/s)
Black tube format (1ml) upper limit (2 s acquisition time)
Background (used for subtraction of sample)	-	39
pAK1-lux	1.1 × 10⁸ ± 1.0 × 10⁷	7,470 ± 136
pCGLS-1	6.2 × 10⁷ ± 1.2 × 10⁷	6,168 ± 167
pXEN-1	1.0 × 10⁸ ± 1.0 × 10⁷	7,016 ± 136
Black tube format (1. 0 ml) lower limit (2 s acquisition time)
Background (used for subtraction of sample)	-	33
pAK1-lux	2.7 × 10⁶ ± 1.0 × 10⁷	278 ± 136
pCGLS-1	1.8 × 10⁶ ± 1.0 × 10⁷	327 ± 136
pXEN-1	5.1 × 10⁶ ± 1.0 × 10⁷	148 ± 141
Item	Bacterial concentration (CFU)	Photonic emissions (RLU/s)
96-well black plate format (100 μl) lower limit (30 s acquisition time)
Background (used for subtraction of sample)	-	6
pAK1-lux	3.8 × 10³ ± 2.8 × 10³	2.0 ± 1.3
pCGLS-1	2.9 × 10³ ± 2.8 × 10³	1.1 ± 1.3
pXEN-1	2.8 × 10³ ± 2.7 × 10³	1.1 ± 1.2

Luminescent Salmonella typhimurium with three different plasmids (pAK1-lux, pXEN-1, and pCGLS-1); upper and lower detection limits for black tube format (2 s acquisition time) and low detection limits for black 96-well plate format (30 s acquisition time). The results below are bacterial concentration means ± standard error of the mean and photonic emissions means ± standard error of the mean.

ISSN: 1471-2180