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Figure 2 | BMC Microbiology

Figure 2

From: A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus

Figure 2

Electromobility shift of mec operator DNA by SA1665. A, Gel shift using biotinylated DNA (6 ng) and crude protein extracts. Lane 1, DNA only control; lanes 2 and 3, DNA incubated with 200 ng and 500 ng of crude protein extract from E. coli BL21 pET28nHis6, respectively; lanes 4 and 5, DNA incubated with 200 ng and 500 ng of crude protein extract from E. coli BL21 pME20, expressing SA1665, respectively. B, Gel shift of biotinylated DNA (6 ng) with purified SA1665 protein. Lane 1, DNA only control; lanes 2–7 DNA incubated with 10, 40, 75, 150, 200 and 250 ng protein, respectively; lane 8, DNA incubated with 250 ng of protein in the presence of a 130-fold excess of unlabelled specific competitor DNA; lane 9, DNA incubated with 250 ng of protein in the presence of unspecific competitor (herring sperm) DNA.

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