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Figure 1 | BMC Microbiology

Figure 1

From: A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus

Figure 1

DNA-binding protein purification assay using mec operator DNA region as a bait. A, Silver stained SDS-polyacrylamide protein gel containing the elutions from DNA-binding protein capture assays performed with either DNA-coated (+) or uncoated (-) streptavidin magnetic beads. One protein band, indicated by the arrow, was only captured by the DNA-coated beads, indicating that it bound specifically to the mec operator DNA. The protein size marker (M) is shown on the left. B, Organisation of the genomic region surrounding SA1665. The regions used to construct the deletion mutants are indicated by lines framed by inverted arrow, which represent the positions of primers used for their amplification. The chromosomal organisation, after deletion of SA1665 is shown beneath. The position of the SA1665 transcriptional terminator, which remained intact after SA1665 markerless deletion is indicated ().

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