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Table 2 PCR detection of excised circular intermediates of the genomic islands GI1 to GI7

From: Genomic island excisions in Bordetella petrii

 

Primer combinations used

Size of the expected PCR product [bp]

PCR product obtained

GI1

GI1–1/GI1–2

1,331

-

GI1*

GI1–2/GI1–3

677

+

GI2

GI2-1/GI2–2

624

+

GI2*

GI2–3/GI2–4

902

-

GI3

GI3–1/GI3–2

967

+

GI1+GI2

GI2–3/GI1–2

1,175

-

GI2+GI3

GI3-2/GI2-2

578

+

GI2*+GI3

GI3-3/GI2–4

494

-

GI1–GI3

GI3-2/GI1–2

720

+

GI4

GI4-1/GI4-2

384

+

GI5

GI5-1/GI5-2

571

-

GI6

GI6-1/GI6-2

850

+

GI7

GI7-1/GI7-2

384

+