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Table 1 Analysis of cell motility of GFP-YopE cells

From: Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideumthrough targeting of multiple Rho family GTPases

 

Control

GFP-YopE

Buffer

  

Speed (μm/min)

7.35 ± 3.62

7.27 ± 3.18

Persistence (μm/min × deg)

2.10 ± 1.25

2.23 ± 1.50

Directionality

0.42 ± 0.24

0.53 ± 0.25

Directional change (deg)

40.01 ± 14.51

38.41 ± 15.52

cAMP gradient

  

Speed (μm/min)

9.02 ± 2.89

8.23 ± 3.08

Persistence (μm/min × deg)

2.94 ± 1.72*

2.83 ± 1.53

Directionality

0.78 ± 0.19*

0.71 ± 0.21*

Directional change (deg)

20.13 ± 10.49*

26.49 ± 12.69*

  1. Time-lapse image series were captured and stored on a computer hard drive at 30 seconds intervals. The DIAS software was used to trace individual cells along image series and calculate motility parameters. Objects whose speed was <2 μm/min were excluded from the analysis. Persistence is an estimation of movement in the direction of the path. Directionality is calculated as the net path length divided by the total path length, and gives 1.0 for a straight path. Directional change represents the average change of angle between frames in the direction of movement. Values are mean ± standard deviation of approximately 50 cells from at least three independent experiments. Control cells are cells of the parental MB35 strain. * P < 0.01 relative to the same strain in buffer (Student's t test).
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BMC Microbiology

ISSN: 1471-2180

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