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Table 2 The targeted genes and PCR primers used for the detection and the differentiation of Cp. abortus, Cp pecorum and C. burnetii.

From: Simultaneous differential detection of Chlamydophila abortus, Chlamydophila pecorum and Coxiella burnetii from aborted ruminant's clinical samples using multiplex PCR

Target gene

Primers name

Primers sequence (5'-3')

Amplified fragment length (bp)

Melting temperature (°C)

pmp 90/91

pmp-F

CTCACCATTGTCTCAGGTGGA

821

64

 

pmp-R821

ACCGTAATGGGTAGGAGGGGT

 

66.3

CPC

Cpc-F

TTCGACTTCGCTTCTTACGC

526

64.3

 

Cpc-R

TGAAGACCGAGCAAACCACC

 

67.4

IS1111a

Trans-1

TATGTATCCACCGTAGCCAGT

687

67.5

 

Trans-2

CCCAACAACACCTCCTTATTC

 

66

  1. The name, the sequence, the target gene and the predicted amplified fragment, as well as the melting temperature are listed.