Figure 3
From: PpoR is a conserved unpaired LuxR solo of Pseudomonas putida which binds N-acyl homoserine lactones
3-oxo-C6-HSL measurement produced by P. putida WCS358 and by the ppoR mutant WCS358PPOR. AHLs were extracted from spent supernatants and levels were measured using a biosensor as described by Steindler et al., [15]. AHL levels were measured using a volume of extract corresponding to an amount of 5 × 108 cfu as described in the Methods section. 3-oxo-C6-HSL level is proportional to β-galactosidase activity (Miller Units); β-gal refers to β-galactosidase; OC6 refers to 0.05 μM of synthetic 3-oxo-C6-HSL used for the experiment and EA refers to ethyl acetate added as control in the experiment. β-galactosidase activity (Miller Units) represented as bars were obtained from one such experiment; similar values were obtained from additional experiments carried out with AHLs extracted independently from P. putida WCS358 and WCS358PPOR strains.