Figure 3

A pstS mutation effects transcription of pigA and smaI via PhoBR. Primer extension analysis was used to measure the level of (A) pigA or (C) smaI transcripts in WT, pstS mutant (ROP2), or pstS, phoB (RBR9) double mutant strains throughout growth in LB. (B) β-Galactosidase activity was measured from a chromosomal pigA::lacZ fusion in an otherwise WT background (NW60), or in pstS (PCF76), phoR (PCF75), phoB (PCF74), pstS, phoR double (PCF78) or pstS, phoB double (PCF77) mutant backgrounds. Activity was assayed from cells grown to early stationary phase in LB.