Application of LSplex for detection of bacterial mixtures from clinical specimens. Hybridization profiles generated by DNA isolated from cotton swab of superficial wound. DNA was labeled prior to hybridization without amplification (green) or after LSplex (red). Each row represents an individual capture probe of the microarray, grouped by species or genus specific regions (see Additional file 2) as indicated in the left column. The boxes represent the positive hybridization signal of bacterial DNA (in color) or absence of hybridization (in white) with individual capture probes. The presence of E. faecium and S. epidermidis on swab was verified by routine microbiological diagnostic procedures.