Figure 3

Expression of the O. tritici ars genes in E. coli AW3110 under control of ptrc promoter. The gel used was a SDS-12% polyacrylamide gel. Lane1, marker proteins, lane2, plasmid pTRC 99A without an insert; lanes 3, 4 and 5, construct parsRDAcbsB in absence of any oxyanion, in presence of 1 mM As(III) and 1 mM Sb(III), respectively; lanes 6, 7 and 8, construct parsDAcbsB without any oxyanion, in presence of 1 mM As(III) and 1 mM Sb(III), respectively.