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Figure 9 | BMC Microbiology

Figure 9

From: Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner

Figure 9

Reactivity of human antibodies with endogenous pgp3 on Western blot. The C. trachomatis L2-infected cell cytosolic preps (L2S100) were precipitated with protein G agarose beads (Pr.G-Beads) bound with anti-pgp3 (mAb clone 2H4) or anti-CPAF (clone 54b) antibodies as indicated on top of the figure. The precipitates were resolved in SDS gels and blotted for reacting with the anti-GSP-pgp3 (pAb; panel a), anti-CPAF (100a; b) or the pooled positive human antiserum (c) in a Western blot as listed along the left of the figure. Lane#1 was loaded with the L2S100 without precipitation as positive antigen control. The corresponding protein bands were indicated on the right of the figure. Note that human antibodies detected both the precipitated endogenous CPAF C- & N-terminal fragments but not pgp3.

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