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Figure 8 | BMC Microbiology

Figure 8

From: Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner

Figure 8

Reactivity of human antibodies with pgp3 fragment fusion proteins. The pgp3 and its fragment fusion proteins were reacted with the pooled human at 1:4,000 dilution (panel e) and mouse antisera at 1:4,000 (d), both produced during live chlamydial infection, and polyclonal antibody (pAb, a) and mAbs (clone 2H4, b; clone 4E6, c) raised by immunizing mice with pgp3 fusion protein as listed along the left of the figure. The corresponding protein bands were indicated on the right of the figure. Note that only the anti-pgp3 fusion protein-raised antibody recognized pgp3 and its many fragments and no other antibodies picked any significant signals.

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