Genetic characterization of clinical and agri-food isolates of multi drug resistant Salmonella enterica serovar Heidelberg from Canada

Andrysiak, Ashleigh K; Olson, Adam B; Tracz, Dobryan M; Dore, Kathryn; Irwin, Rebecca; Ng, Lai-King; Gilmour, Matthew W

doi:10.1186/1471-2180-8-89

Table 1 Bacterial strains used in this study and PCR-based replicon typing and resistance gene characterization for S. Heidelberg and E. coli bla_cmy-2 plasmids.

From: Genetic characterization of clinical and agri-food isolates of multi drug resistant Salmonella enterica serovar Heidelberg from Canada

Strain^a	Year	PT^b	Province^c	Source	Resistance Phenotype^d	Resistance Genotype^e	bla_cmy-2 plasmid mediated resistance determinants^e	Rep^h
01–7169	2001	29	ON	Human stool	Amp, Chl, Cro, Gen, Kan, Str, Sxt, Tio	aadA2, strA, sul1	n/a	n/a
02–0102	2001	11a	ON	Human stool	Chl, Str, Sul	aadA1, aadA2	n/a	n/a
02–2339^fg	2002	19	ON	Human stool	Sensitive	n/a	n/a	n/a
02–4660^fg	2002	19	ON	Human stool	Amc, Amp, Chl, Fox, Str, Sxt, Tet, Tio	bla_cmy-2, dhfrA1, floR, strA, sul1, tetA	bla_cmy-2, dhfRA1, floR, strA, sul1, tetA	A/C
02–5785^f	2002	AT02–5785	ON	Human stool	Amc, Amp, Chl, Fox, Gen, Kan, Smx, Str, Tet, Tio	bla_cmy-2, floR, strA, sul1, tetA	n/i	n/a
02–5980	2002	29	QC	Human blood	Amc, Amp, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
03–0262^fg	2003	41	QC	Human blood	Amc, Amp, Fox, Cep, Cro, Tio	bla _cmy-2	bla _cmy-2	I1
03–0845^f	2003	29	ON	Human stool	Amc, Amp, Fox, Cep, Chl, Gen, Kan, Smx, Str, Tet, Tio	aadB, bla_cmy-2, cmlA, strA, sul1, tetA	aadB, bla_cmy-2,cmlA, strA, sul1, tetA	A/C
03–3012	2003	4	QC	Human blood	Amc, Amp, Cep, Cro, Fox, Tio	bla _cmy-2	bla_cmy-2	I1
03–4601	2003	AT03–4601	QC	Human blood	Amc, Amp, Cep, Chl, Cro, Fox, Tio, Smx, Str, Tet, Tio	bla_cmy-2, floR, strA, tetA	bla_cmy-2, floR, strA, tetA	A/C; I1
03–4690	2003	32	ON	Human stool	Amc, Amp, Cep, Cro, Fox, Gen, Str, Tet, Tio	bla_cmy-2, strA, tetB	bla _cmy-2	I1
03–7402^fg	2003	29	ON	Human blood	Amc, Amp, Cep, Cro, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
04–0346^fg	2004	54	QC	Human stool	Amc, Amp, Cep, Chl, Cro, Fox, Smx, Str, Tet, Tio	bla_cmy-2, floR, strA, tetA	bla_cmy-2, floR, tetA	A/C
04–1511	2004	41	ON	Human stool	Amc, Amp, Cep, Fox, Smx, Str, Tet, Tio	bla_cmy-2, strA, tetA	bla _cmy-2	I1
04–3194	2004	29	ON	Human blood	Amc, Amp, Cro, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
04–3293	2004	29a	QC	Human blood	Amc, Amp, Cro, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
04–4717	2004	29	QC	Human blood	Amc, Amp, Cro, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–5435	2004	29	QC	Human blood	Amc, Amp, Cro, Fox, Tet, Tio	bla _cmy-2	bla _cmy-2	I1
04–5511^fg	2004	41	ON	Human stool	Sensitive	n/a	n/a	n/a
05–4260	2001	29	ON	Bovine passive	Amc, Amp, Cep, Fox, Tio	n/d	n/a	n/a
05–4262^fg	2001	Untypable	AB	Chicken passive	Amc, Amp, Cep, Chl, Fox, Gen, Kan, Smx, Str, Tet, Tio	bla_cmy-2, floR, strA, sul1, tetA	bla_cmy-2, floR, strA, sul1, tetA	A/C; I1
05–4263^g	2001	Untypable	ON	Chicken passive	Amc, Amp, Cep, Fox, Tet, Tio	bla_cmy-2, tetB	bla _cmy-2	I1
05–4264	2002	Atypical	ON	Bovine passive	Amc, Amp, Cep, Chl, Fox, Gen, Kan, Str, Sxt, Tet, Tio	aadA1, bla_cmy-2, dhfRA1, floR, strA, sul1, tetA	n/i	n/a
05–4269^fg	2003	29	ON	Turkey passive	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4272	2003	29	QC	Chicken retail	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4275	2003	32	QC	Chicken retail	Amc, Amp, Cep, Fox, Gen, Str, Tet, Tio	bla_cmy-2, strA, tetB	bla _cmy-2	I1
05–4277	2003	29	QC	Chicken retail	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4287	2003	29	ON	Chicken abattoir	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4294	2004	29	ON	Chicken retail	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4299	2004	29	ON	Chicken abattoir	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	Unknown
05–4316^f	2004	29	PE	Bovine passive	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
05–4354^fg	2004	41	QC	Porcine passive	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	Unknown
05–4355	2004	29	QC	Porcine passive	Amc, Amp, Cep, Fox, Tio	bla _cmy-2	bla _cmy-2	I1
00–5440	2000	29	AB	Human	Sensitive	n/a	n/a	n/a
05–1147	2005	29	QC	Human	Sensitive	n/a	n/a	n/a
539	unknown	n/d	ON	Chicken rinse	Sensitive	n/a	n/a	n/a
564	unknown	n/d	ON	Egg yolk mix	Sensitive	n/a	n/a	n/a
1170	unknown	n/d	ON	Cocoa beans	Sensitive	n/a	n/a	n/a
S-467	1948	n/d	BC	Human	Sensitive	n/a	n/a	n/a
E. coli 830	2004	n/d	AB	Chicken abattoir	Amc, Amp, Cep, Fox, Gen, Smx, Str, Tet, Tio	aadA1, bla_cmy-2, sul1	aadA1, bla _{cmy 2}	Unknown
E. coli 831	2004	n/d	QC	Chicken abattoir	Amc, Amp, Chl, Fox, Kan, Smx, Str, Tet, Tio	aadA1, bla_cmy-2, floR, strA, sul1, tetA	aadA1, bla_cmy-2, floR, strA, tetA	A/C
E. coli 832	2004	n/d	QC	Porcine abattoir	Amc, Amp, Cep, Chl, Fox, Smx, Str, Tet, Tio	bla_cmy-2, floR, strA, tetA	bla_cmy-2, strA, tetA, floR	A/C
E. coli 833	2004	n/d	ON	Chicken abattoir	Amc, Amp, Cep, Fox, Gen, Smx, Str, Tio	aadA1, bla_cmy-2, floR, sul1	aadA1, bla _{cmy 2}	I1
E. coli 834	2004	n/d	QC	Bovine retail	Amc, Amp, Cep, Fox, Tio	bla_cmy-2, bla_cmy-2	bla _{cmy 2}	I1

^a All strains are S. Heidelberg unless identified otherwise
^b PT, phage type; n/d, not determined
^c AB, Alberta; BC, British Columbia; PE, Prince Edward Island; QC, Quebec; ON, Ontario
^d Amc, amoxicillin-clavulanic acid; Amp, ampicillin; Fox, cefoxitin; Tio, ceftiofur; Cro, cefriaxone; Cep, cephalothin; Chl, chloramphenicol; Gen, gentamycin; Kan, kanamycin; Nal, nalidixic acid; Str, streptomycin; Sul, sulfamethoxazole; Tet, tetracycline; Smx, sulfizoxazole; Sxt, trimethoprim-sulfamethoxazole. n/a, not applicable.
^e aadA1, streptomycin/spectinomycin adenyltransferase; aadB, aminoglycoside adenyltransferase; cmlA, chloramphenicol/florphenicol efflux; dhfR, dihydrofolate reductase; floR, efflux; strA, streptomycin phosphotransferase; sul1, dihydropteroate synthase; tetA, efflux; tetB, efflux; bla_cmy-2, beta-lactamase. n/a, not applicable; n/i, plasmid not isolated; n/d, not determined.
^f CGH were conducted on these isolates
^g DNA sequence-based typing was performed on these isolates
^h n/a, not applicable

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ISSN: 1471-2180

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