Formate hydrogenlyase in the hyperthermophilic archaeon, Thermococcus litoralis

BMC Microbiology

Table 2 Properties of the deduced gene products of the T. litoralis fdh-mhy operon.

Gene product	molecular mass (kDa)	predicted membrane helices [52]	properties	proposed function	homologues of			% sequence homologies
					E.coli FHL-1	E. coli Nuo	P. abyssi Hase-4	T.l./E.c. FHL-1 [Ref.]	T.l./P.a. [Ref.]
FdhA	71.6	2	molybdopterin cofactor binding motif	formate-dehydrogenase α subunit	Fdh-H		Fdh α	29 [9]	33 [12]
FdhB	18.5	0	2 [4Fe4S] binding site	e^- transport protein	HycB		Fdh FeS	33 [9]	55 [12]
MhyC	49	13	NuoL type membrane protein	membrane anchor, proton translocation		NuoL	Hase-4 subunit		53 [12]
MhyD	65.7	15	NuoN type membrane protein	membrane anchor, proton translocation	HycC	NuoN	Comp. B	28 [9]	36 [12]
MhyE	32	5	NuoH type membrane protein	membrane anchor, proton translocation	HycD	NuoH	comp. C	32 [9]	52 [12]
MhyF	62.4	0	conserved CxxC and DPCxxCxxH/R motives	hydrogenase large subunit	HycE	NuoD	comp. G	46 [9]	55 [12]
MhyG	18.7	0	2 [4Fe4S] binding site	e^- transport protein	HycF	NuoI	comp. B	36 [9]	51 [12]
MhyH	29.6	1	1 [4Fe4S] binding site	hydrogenase small subunit	HycG	NuoB	comp. I	37 [9]	51 [12]

ISSN: 1471-2180