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Table 3 Proteins identified in the OMV fraction of cultures grown in XVM2 medium.

From: Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris

Section

Protein

Acc.No.

MW(cal)

Size range

Coverage (ESI-MS/MS)

Score (ESI-MS/MS)

Coverage (MALDI)

Score (MALDI)

1

TonB-dependent receptor with signalP

AAM 42139

100858

80–150

8

311

10

134

 

XadA1 Xanthomonas adhesion XadA

AAM39974

220132

 

7

35

-

-

 

HrpA ATP-dependent RNA helicase

AAM42217

151602

 

6

32

8

92

 

RhsD (RhsD protein) with signalP

AAM39448

164320

 

5

32

7

66

2

FpvA TonB-dependent outer membrane ferripyoverdine receptor with signalP

AAM42628

81083

60–100

9

162

12

118

 

TonB-dependent outer membrane receptor

AAM41663

91909

 

4

61

8

86

 

HrpF (HrpF protein)

AAM40515

98836

 

6

35

14

84

 

AvrBs2 (avirulence protein)

AAM39371

78471

 

10

33

-

-

 

FasD outer membrane usher protein

AAM40677

86678

 

6

30

-

-

3

OmpA family outer membrane protein with signalP

AAM40245

39338

40–60

28

505

21

97

 

XynB exported xylan 1,4-beta-xylosidase with signalP

AAM43196

58311

 

5

34

-

-

 

UptE Outer membrane protein with signalP

AAM39911

31591

 

15

33

19

56

 

AvrBs1 avirulence protein AvrBs1

AAM41388

49789

 

8

31

13

68

4

OmpA family outer membrane protein with signalP

AAM40245

39338

30–40

18

113

20

102

 

Egl exported cellulase with signalP

AAM42791

52209

 

4

39

9

86

 

HrcU HrcU protein

AAM40528

38694

 

8

30

12

57

 

HrpX AraC-type transcriptional regulator HrpX

AAM40465

52250

 

6

30

18

83

 

HrcN HrcN protein

AAM40534

47759

 

12

29

10

58

5

OmpW3 OmpW family outer membrane protein

AAM42791

23494

25–30

7

55

7

67

 

HrpB4 HrpB4 protein

AAM40532

22919

 

4

36

8

59

6

OmpW3 OmpW family outer membrane protein

AAM42791

23494

20–25

7

48

7

71

 

XpsH General secretion pathway protein

AAM39979

18208

 

20

47

17

61

7

Putative exported protein with signalP

AAM40594

10647

10–20

15

245

21

117

 

UptD outer membrane protein with signalP

AAM39910

17116

 

12

27

17

74

  1. The section indicates the gel-section excised from the corresponding SDS-PAGE. The values indicated under MW(cal) gives the calculated molecular weight of the identified proteins. The size range relates the excised gel-section with regard to the molecular weight standard on the SDS-PAGE. Score (MOWSE-score) and coverage (sequence coverage) relate to the two approaches (ESI-MS/MS or MALDI-TOF-MS) utilized for the identification of the proteins on the SDS-PAGE.